人体血液循环模拟系绞�/strong>＋�span>VenaFlux 微流体细胞研究系� Automated microfluidic platform是提供在活细胞水平上微流体活性筛选的完整解决方案。操作简单，分析效率高，可大大提高药品的开发和筛选效率、�/span>

VenaFlux 系统�?*个能进行连续流体细胞分析的半自动化微流体分析系统。配合使用Cellix生物芯片和细胞分析软件，VenaFlux 系统可检测粘附到微毛细管上的细胞，微毛细管中覆盖抗体或者培养有内皮细胞，模拟生理流动产生一定的剪切力、�/p>

VenaFlux 系统操作简单，可减少耗时耗钱的动物模型实验，获得特异性强，准确性高，重复性好的结果、�/span>

VenaFlux流体细胞分析仪产品特点：

广泛使用于各种细胞悬浮液：�/span>

生物芯片可选择用光学显微镜做细节研究；

微流体泵可方便的控制流体剪切力在0.05-200 Dyne/cm2范围内；

在分析中可调整剪切力：�/span>

流体速度可控制在pL/min到mL/min：�/span>

可做nL到mL 的样品分析；

使用DucoCell 分析软件（DucoCell Analysis Software），可简单快速的调节好显微镜的各项参数；

统计分析生成的图表（如：细胞粘附性VS.剪切力）可方便的导入Excel文档：�/span>

电子机械臂移动生物芯片，清洗细微组件和稀释细胞样品；

VenaFlux系统的软件同步调节泵，翻译阶段筛选，图像采集，样品移动和流体控制，各项操作界面均是用户友好型，系统先进又易于操作：�/span>

主要配置方案９�/strong>

一、Partial Platform -STARTER-1.0９�/span>

1、Mirus Nanopump 1.0

2�? pack VenaEC biochips

3�? pack Vena8 biochips

4、Frame for VenaEC biochips

5、DucoCell Software ver 1.1

二、Microfluidic SP1.0 (Basic full Package)-MICROSP-1.0９�/span>

1、arl Zeiss Axiovert 40 microscope� CCD Deltapix DP200 camera� Dell PC� Microscope cage incubator

2、Mirus Nanopump 1.0 inc. VenaFluxAssay software

3�? pack Vena8 biochips

4� 1 pack VenaEC biochips

5、DucoCell Software ver 1.1

三、CMicrofluidic SP1.1-MICROSP-1.1９�/span>

1、Carl Zeiss Axiovert 40 CFL microscope� CCD Deltapix DP450 camera� Dell PC� Microscope cage incubator� Fluorescent and brightfield shutters �

2、Mirus Nanopump 1.0 inc. VenaFluxAssay software

3�? pack Vena8 biochips

4�? pack VenaEC biochips

5、DucoCell Software ver 1.1

四、Microfluidic SP1.2-MICROSP-1.2:

1、Carl Zeiss Axiovert 40 CFL microscope� CCD Deltapix DP450 camera� Dell PC� Microscope cage incubator� Fluorescent and brightfield shutters，motorised

stage

2、Mirus Nanopump 1.0 inc. VenaFluxAssay software

3�? pack VenaEC biochips

4�? pack Vena8 biochips

5、DucoCell Software ver 1.1

五、VenaFlux-VENAFLUX-1.0９�/span>

1、Carl Zeiss Axiovert 40 CFL microscope� CCD Deltapix DP450 camera� Dell PC� Microscope cage incubator� Fluorescent and brightfield shutters

2、VenaFluxAssay software plus automatic image capture

3、Robotic sample handler

4、Mirus Nanopump 1.1 inc. 8-way Manifold

5�? pack VenaEC biochips

6�? pack Vena8 biochips

7、Frame for VenaEC biochips

8、DucoCell Software ver 1.1

主要技术参数：

Microscope Axiovert 40:

Contrast option: Brightfield� Phase� Varel.

Objectives: 10x LD A-Plan� 20x LD A-Plan� 32x CP-Achromat� 40x LD A-Plan

Eyepieces: E-PL 10x/20 Br.� 10x/20 Br. foc.

Stage: Vena8 biochip compatible stage holder� Eppendorf tubes holder.

Mirus 1.0 Nanopump

Specifications: Shear Stress Range of 0.05�?0 dyne/cm2�

Volumetric Flow Rates 100nL�?0��L/minute (100��L syringe)�

Sample Volume Increments Freely adjustable Linear Velocity Range of 10 ��m/s to 10 cm/s;

Flow Direction Reversible;

Sample Volume Aspiration Accuracy ��1% ;

Shear Stress Accuracy ��0.5%;

Sample Volume Aspiration Precision < 1%.

Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.

Microscope Cage incubator:

Capabilities: Temperature control �� 0.10C� CO2 and humidity module.

Options: Temperature monitoring software module� black panels for use in fluorescent microscopy.

Deltapix DP 200 USB 2.0 camera

Specifications: 1.3 Mega Pixels (1�?80 x 1�?24)� Color CDD� 1/2" format� Pixel 5.2 ��m x 5.2 ��m�

Resolution: 15 FPS @ 1�?80 x 1�?24 pixels; 24 FPS @ 1�?04 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.

Dell Optiplex 745 PC computer

Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO;

Objective Imaging stage control hardware; 19� LCD monitor.

Windows XP Professional SP2;

DeltaPix Viewer Software;

FlowAssay and DucoCell software preinstalled.

FlowAssay Software.

Capabilities: Execution of preset protocols.

Intuitive stepwise protocol interface with steps hierarchy and status control.

DucoCell software

Specifications: Automatic Counting and Detection;

Morphological parameters analyzed automatically(Area� diameter� form-factor� ellipticity� eccentricity� orientation� perimeter� asymmetry� cell centroid coordinates� elliptical axis);

Cell sorting and filtering;

Ability to analyze sub-populations based on inclusion/exclusion of morphological parameters.

Data may be exported to Excel spreadsheet incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress).

File Formats analyzed*.jpg� *.jpeg� *.tif� *.tiff� *.bmp� *.gif� *.png.

Vena8 biochips pack of 10

Specifications: Minimum Sample Volume 3 ��L;

Maximum Sample Volume 100 ��L; Shear Stress Range of 0.05� 20 dyne/cm2� steps of 0.05dyne/cm2.

Number of channels per biochip is 8;

Volume of each channel is 0.8��L.

Dead volume at input port is 0.1 ��L. Vena8 biochip dimensions are 400 ��m (W) x 100 ��m (D) x 20 mm(L).

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