閰嶅埗涓ょ浉鍒嗘暎浣撶殑涓€涓叡鍚岀洰鏍囨槸鏈€澶ч檺搴﹀湴鎻愰珮浜у搧鐨勭ǔ瀹氭€ф垨淇濊川鏈熴€傛偓娴恫鍜屼钩娑查兘鏄姝ゃ€傜矑寰勫拰琛ㄩ潰鐢佃嵎锛坺eta 鐢典綅锛夐兘鏄奖鍝嶆偓娴恫绋冲畾鎬х殑閲嶈鐗╃悊鐗规€с€傛湰搴旂敤璇存槑瑙ｉ噴浜嗗浣曚娇鐢ㄧ矑寰勫拰 zeta 鐢典綅娴嬮噺鏉ユ彁楂樺垎鏁ｇǔ瀹氭€с€侟/span>

鍏抽敭璇岤Zeta鐐逛綅;Nicomp鐢典綅;绮掑緞

1 浠嬬粛

鎮诞娑诧紙鍥轰綋/娑蹭綋锛夊拰涔虫恫锛堟恫浣?娑蹭綋锛夐兘鏄袱鐩稿垎鏁ｄ綋鐨勪緥瀛愩€傛憾鍓傛槸杩炵画鐩革紝棰楃矑鎴栦钩娑叉恫婊存瀯鎴愬垎鏁ｇ浉銆傚湪绋冲畾鐨勫垎鏁ｄ綋涓紝鍒嗘暎鐩镐細闅忕潃鏃堕棿鐨勬帹绉讳繚鎸佷竴鑷寸殑绮掑緞骞朵繚鎸佹偓娴姸鎬併€備笉绋冲畾鐨勪钩娑插彲浠ラ殢鐫€娌圭浉鈥滀钩鍖栤€濆埌椤堕儴鑰岀浉鍒嗙锛屾垨鑰呬钩娑叉恫婊寸敱浜庤仛闆嗘垨濂ユ柉鐗圭摝灏斿痉鐔熷寲鑰岀殑浣滅敤锛岄殢鏃堕棿鎺ㄧЩ鑰屽鍔犮€備笉绋冲畾鐨勬偓娴恫浼氱诞鍑?鑱氶泦锛岀劧鍚庢矇闄嶅埌搴曢儴锛屽鍥 1 鎵€绀恒€侟/p>

鍥 1. 绋冲畾鎬т笌涓嶇ǔ瀹氭€?/p>

鍒涘缓绋冲畾鐨勫垎鏁ｄ綋娑夊強鎺у埗杩炵画鐩稿拰鍒嗘暎鐩哥殑鍖栧鍜岀墿鐞嗘€ц川銆傞€氳繃閫夋嫨涓嶅悓鐨勮〃闈㈡椿鎬у墏鍜屾祿搴︺€佹敼鍙樼洂娴撳害銆佹帶鍒秔H鍊间互鍙婃墍鏈夎繖浜涘洜绱犵殑缁勫悎锛屽彲浠ヤ紭鍖栬繛缁浉鐨勫寲瀛︽€ц川銆傞€氳繃鍦ㄨ〃闈㈡坊鍔犺仛鍚堢墿娑傚眰锛堢┖闂寸ǔ瀹氾級銆佸鍔犺〃闈㈢數鑽凤紙闈欑數绋冲畾锛変互鍙婁袱鑰呯殑缁撳悎锛屽彲浠ヤ娇鍒嗘暎鐩告洿鍔犵ǔ瀹氥€侟/p>

褰撻绮掕〃闈㈡病鏈夌數鑽锋椂锛岄绮掑彲浠ヨ冻澶熺揣瀵嗗湴鐩镐簰鎺ヨ繎锛屼互鑷充簬娌℃湁灞忛殰闃绘瀹冧滑鑱氶泦锛屼粠鑰岄檷浣庡垎鏁ｇǔ瀹氭€с€備絾鏄紝濡傛灉琛ㄩ潰涓婂瓨鍦ㄨ冻澶熺殑鐢佃嵎锛岀矑瀛愬氨浼氬儚纾侀搧涓€鏍风浉浜掓帓鏂ワ紝骞朵笖姘歌繙涓嶄細瓒冲鎺ヨ繎浠ヨ仛闆嗭紝濡傚浘2鎵€绀恒€侟/p>

鍥?.绮掑瓙鍚稿紩鑱氶泦锛堝乏锛夛紝绮掑瓙鎺掓枼鍒嗙锛堝彸锛堻/p>

浠庢祴閲忕殑瑙掑害鏉ョ湅锛屼娇鐢▃eta鐢典綅鍒嗘瀽鏉ヨ〃寰佽〃闈㈢數鑽枫€倆eta 鐢典綅鏄窛绂婚绮掍笉杩滅殑鐢典綅锛屽崟浣嶄负 mV锛屽鍥 3 鎵€绀恒€ 娴嬮噺鏄€氳繃瀵规偓娴恫鏂藉姞鐢靛満骞堕€氳繃鐢垫吵鍏夋暎灏勬娴嬮绮掔殑杩愬姩鏉ヨ繘琛岀殑銆傜矑瀛愮Щ鍔ㄧ殑鏂瑰悜鍐冲畾浜嗙鍙凤紙+鎴?锛夛紝鑰岄€熷害鍐冲畾浜嗙數鑽风殑澶у皬銆傚彲浠ラ€氳繃妫€娴嬮绉伙紙鐩镐綅澶氭櫘鍕掞級鎴栫浉绉伙紙鍏夋暎灏勭浉浣嶅垎鏋愭垨 PALS锛夋潵鍒嗘瀽杩愬姩銆侟/p>

鍥?.Zeta鐢典綅

zeta 鐢典綅娴嬮噺鐨勪富瑕佺粨鏋滄槸鐢垫吵杩佺Щ鐜 渭锛岀劧鍚庝娇鐢ㄤ互涓嬪叕寮忚绠 zeta 鐢典綅锛欬/span>

zeta 鐢典綅鏄鐢佃嵎杩樻槸璐熺數鑽峰苟涓嶉噸瑕侊紝閲嶈鐨勬槸缁濆澶у皬銆傝緝楂樼殑 zeta 鐢典綅鍊兼槸绋冲畾鎬ч珮鐨勬寚鏍囥€備笉鍚岀绫荤殑鍒嗘暎浣撻渶瑕佷笉鍚岀殑鐢佃嵎鍊兼潵澧炲己绋冲畾鎬э紝濡傚浘4鎵€绀猴紙浠呯矖鐣ユ寚鍗楋級銆侟/span>

鍥?.Zeta 鐢典綅绋冲畾鎬ф寚鍗桙/p>

閫氳繃鏀瑰彉 zeta 鐢典綅鏉ユ彁楂樼ǔ瀹氭€ч€氬父鍙灏忛绮掞紙骞冲潎灏哄浣庝簬 1 渭m锛夊緢閲嶈锛屽浜庝钩娑茶€岃█锛岃繖涓昂瀵稿彲鑳戒細鏇村ぇ銆侟/p>

2 绛夌數鐐癸紙IEP锛堻/strong>

鍒嗘暎浣撶殑绛夌數鐐 锛圛EP锛 鏄 zeta 鐢典綅绛変簬闆舵椂鐨 pH 鍊 1 銆傝繖鏄繘琛 zeta 鐢典綅娴嬮噺鐨勫父瑙佸師鍥狅紝鍥犱负 IEP 鎸囩ず鍙兘瀵艰嚧鍒嗘暎涓嶇ǔ瀹氱殑琛ㄩ潰鍖栧鏉′欢銆傝繖涓蹇靛鍥 5 鎵€绀恒€侟/p>

鍥?.绛夌數鐐更/p>

3 缁撴灉锛氶鍝佷钩娑秉/strong>

閫氳繃鐢╬H 6.8鑷?.2鐨勫急閰告淮瀹氭潵鏀瑰彉椋熷搧涔虫恫鐨刾H鍊笺€備娇鐢 PALS 鎶€鏈湪姣忎釜pH鍊间笅杩涜杩涜 7 娆℃祴閲忥紙鏂藉姞鐢靛満 = 4 v/cm锛夛紝骞舵姤鍛婂钩鍧囧€笺€倆eta 鐢典綅涓 pH 鍊肩殑鍏崇郴鍥惧鍥 6 鎵€绀恒€侟/p>

鍥?.Zeta 鐢典綅涓 pH 鍊肩殑鍏崇郴

涔虫恫鏍峰搧鍦╬H 6.5鏃剁殑绮掑害鍒嗗竷濡傚浘7鎵€绀猴紝鍦╬H 3.5涓嬬殑绮掑緞鍒嗗竷濡傚浘8鎵€绀恒€傝娉ㄦ剰锛屽湪杈冧綆鐨刾H鍊间笅锛屾恫婊村昂瀵革紙302 鈥 496 nm锛夋€ュ墽澧炲姞锛岃〃鏄庝钩娑叉鍦ㄤ笉绋冲畾銆侟/p>

鍥?.pH 6.5 鏃剁殑绮掑緞锛寊eta 鐢典綅 = -34 mV

鍥?.pH 3.5 鏃剁殑绮掑緞锛寊eta 鐢典綅 = -9.8 mV

4 缁撴灉锛氭哀鍖栭攲鎮诞娑秉/strong>

璐拱浜嗘哀鍖栭攲 锛圸nO锛 鈥滅撼绫崇矇鏈€濓紙Sigma Aldrich锛?544906锛夛紝浠ョ爺绌 pH 鍊煎 zeta 鐢典綅鍜岀矑寰勭殑褰卞搷銆傛墍鏈夋牱鍝佸潎閫氳繃鐢 Igepal CA-630 闈炵瀛愯〃闈㈡椿鎬у墏鍒嗘暎骞朵娇鐢ㄨ秴澹版帰澶磋繘琛 3 鍒嗛挓鐨勮秴澹版尝鍒跺.2 pH 鍊 6.9 鍜 11.2 鐨 zeta 鐢典綅鍊煎鍥 9 鍜屽浘 10 鎵€绀恒€傝娉ㄦ剰锛屽嵆浣挎帴杩戦浂锛屼篃浼氫骇鐢熼潪甯哥ǔ瀹氱殑 zeta 鐢典綅缁撴灉銆侟/p>

鍥 9.pH 6.9 鏃 ZnO 鐨 Zeta 鐢典綅

鍥 10.pH 11.2 鏃 ZnO 鐨 Zeta 鐢典綅

鍥?1鏄剧ず浜嗙矑寰勭殑鍙樺寲锛屼粠鍗曞嘲鍒嗗竷锛坧H 6.9鏃跺嘲鍊间负198 nm 钃濊壊宄帮級鍒板弻宄板垎甯冿紙pH 11.2鏃跺嘲鍊间负204鍜?73 nm 绮夌孩鑹插嘲锛夈€傚綋 zeta 鐢典綅鎺ヨ繎闆舵椂锛屾偓娴恫涓嶇ǔ瀹氬苟寮€濮嬭仛闆嗐€侟/p>

鍥 11.pH 鍊间负 6.9锛堣摑鑹诧級鍜 11.2锛堢矇绾㈣壊锛夋椂鐨 ZnO 绮掑緞

5 鍙傝€傸/strong>

1 Entegris Application Note, Isoelectric Point (IEP)

2 Entegris Technical Note, DLS Sample Preparation