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涓嶅悓绉嶇被DNA鐨凾m鍊间笉鍚岋細G-C 鐨勫惈閲忚秺楂? Tm 瓒婇珮锛堢敱浜庨笩鍢屽懁-鑳炲槯鍟讹紙G鈮锛夋牳鑻烽吀涔嬮棿鏈?涓阿閿紝鑰岃吅鍢屽懁-鑳歌吅鍢у暥锛圓=T锛変箣闂存湁2涓阿閿紝G鈮鏍歌嫹閰歌В绂绘墍闇€鑳介噺澶т簬A=T纰卞熀瀵规墍闇€鑳介噺銆傦級, 鐢盩m 鍊煎彲鎺ㄧ畻鍑篏C鍚噺銆傚叾缁忛獙鍏紡涓国 ( G-C)% = ( Tm - 69.3 ) 脳2.44
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鍥撅細260nm澶勯矐楸肩簿DNA鐨勭啍瑙ｆ洸绾军/span>锛堟渚嬫潵婧愭鐗瑰嫆鍏紬鍙凤級
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姊呯壒鍕?鎵樺埄澶氱传澶栧彲瑙佸垎鍏夊厜搴﹁+閰稵甯曞皵璐存帶娓╃郴缁熸柟妗堬細

姊呯壒鍕?鎵樺埄澶氱传澶栧彲瑙佸垎鍏夊厜搴﹁+鍔宠揪锛圠AUDA锛夋按娴存俯鎺х郴缁熻仈鐢ㄦ柟妗堬細