鏈枃閫氳繃浠嬬粛椹皵鏂囧笗绾崇绾崇背绮掑害鍙婄數浣嶄华Zetasizer Ultra鐢ㄤ簬鎱㈢梾姣掕浇浣撻绮掔矑寰勫強杞戒綋婊村害琛ㄥ緛鐨勫疄楠岃缃強妫€娴嬬粨鏋溿€傝鎮ㄥ揩閫熷疄鐜版參鐥呮瘨杞戒綋锛圠V锛夊叧閿川閲忓睘鎬х殑璇勪及銆侟/span>

鎱㈢梾姣掕浇浣擄紙Lentiviral vector, LV锛夋槸鍦℉IV-1鐥呮瘨鍩虹涓婃敼閫犺€屾垚鐨勭梾姣掕浇浣撶郴缁燂紝鍙互灏嗗婧愬熀鍥犳垨澶栨簮鐨剆hRNA鏈夋晥鍦版暣鍚堝埌瀹夸富鏌撹壊浣撲笂锛屼粠鑰岃揪鍒版寔涔呮€ц〃杈剧洰鐨勫簭鍒楃殑鏁堟灉銆傚彲鏈夋晥鍦版劅鏌撶缁忓厓缁嗚優銆佽倽缁嗚優銆佸績鑲岀粏鑳炪€佽偪鐦ょ粏鑳炪€佸唴鐨粏鑳炪€佸共缁嗚優绛夊绉嶇被鍨嬬殑缁嗚優锛屼粠鑰岃揪鍒拌壇濂界殑鐨勫熀鍥犳不鐤楁晥鏋溿€傚浜庝竴浜涜緝闅捐浆鏌撶殑缁嗚優锛 濡傚師浠ｇ粏鑳炪€佸共缁嗚優銆佷笉鍒嗗寲鐨勭粏鑳炵瓑锛屼娇鐢ㄦ參鐥呮瘨杞戒綋锛岃兘澶уぇ鎻愰珮鐩殑鍩哄洜鎴栫洰鐨剆hRNA鐨勮浆瀵兼晥鐜囷紝涓旂洰鐨勫熀鍥犳垨鐩殑shRNA鏁村悎鍒板涓荤粏鑳炲熀鍥犵粍鐨勫嚑鐜囧ぇ澶у鍔狅紝鑳藉姣旇緝鏂逛究蹇嵎鍦板疄鐜扮洰鐨勫熀鍥犳垨鐩殑shRNA鐨勯暱鏈熴€佺ǔ瀹氳〃杈俱€侟span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; visibility: visible; box-sizing: border-box !important; overflow-wrap: break-word !important;">锛堥儴鍒嗗唴瀹规潵鑷櫨搴︾櫨绉戯級

鎵€浠ワ紝鍦ㄤ綋澶栧疄楠屽強浣撳唴瀹為獙鐨勭爺绌朵腑鎱㈢梾姣掕浇浣擄紙LV锛堻span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box; overflow-wrap: break-word !important; text-indent: 2em; letter-spacing: 0px;">涓庤吅鐥呮瘨锛圓d锛夊拰鑵虹浉鍏崇梾姣掞紙AAV锛夊悓涓轰富娴佺殑鐥呮瘨杞戒綋绯荤粺銆傚叾棰楃矑绮掑緞绾︿负90-120nm銆侟/span>

鍦ㄦ參鐥呮瘨杞戒綋锛圠V锛夌殑鐢熶骇宸ヨ壓涓紝鏈夋棤鍥㈣仛浣 锛坅ggregate锛夛紝浠ュ強杞戒綋婊村害锛坱iter锛夌殑楂樹綆鏄噸鐐硅€冨療鐨勫叧閿川閲忓睘鎬э紙CQAs锛夈€俍etasizer Ultra绾崇背绮掑害浠€氳繃瀵筁V棰楃矑鐨勭矑寰勫強杞戒綋婊村害鐨勮〃寰侊紝蹇€熷疄鐜癈QAs鐨勬祴閲忋€侟/p>

Zetasizer Ultra 绾崇背绮掑害鐢典綅浠?/p>

瀹為獙鏂规硶璁惧畾

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鍒嗘瀽缁撴灉

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鍥? LV鏍峰搧鐨勫厜寮虹矑寰勫垎甯冨浘

鍥? LV鏍峰搧鐨勮浇浣撴淮搴?/span>

姝ゅ锛岄櫎浜嗗熀浜嶮ADLS鎶€鏈緱鍒扮殑棰楃矑鐨勫噯纭矑寰勫垎甯冨浘锛屾垜浠繕寰楀埌瀵瑰簲灏哄鐨勮浇浣撴淮搴︿俊鎭紙鍥?锛夈€傚彲浠ョ湅鍒癓V鍗曚綋鐨勯绮掓祿搴︾害1x10¹²涓绮?mL锛屽洟鑱氫綋棰楃矑娴撳害绾︿负1x10¹⁰涓绮?mL锛屼粎涓哄崟浣撶殑1%銆傚崟浣撳拰鑱氶泦浣撴祿搴︾浉宸緝澶х殑鎯呭喌涓嬶紝Zetasizer浠嶅彲寰堝ソ鐨勫尯鍒嗗崟浣撳拰鑱氶泦浣撱€侟span style="margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; text-indent: 2em; letter-spacing: 0px;">

鐐瑰嚮鎷撳睍闃呰锛欬/span>Zetasizer鐢ㄤ簬rAAV棰楃矑绮掑緞鍙婅。澹虫淮搴?/span>