
2025/03/28 阅读?44
方案摘要
一、基础公式与组件定么/h4>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">光学放大倍数计算/p>
物镜?×?0×?0×?0×?00×(标于物镜筒,如“Plan 40×/0.65”表?0倍物镜):/p>
目镜?0×?5×(标于目镜边缘,如“WF10×/18mm”):/p>
中间附件?.5×(摄像适配器)?.5×(分光棱镜)等、/p>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">标准公式:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">典型组件倍率:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">数码成像系统放大倍数修正/p>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">显示器放大倍率:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">总数码放大倍数:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">光学放大倍数计算/p>
物镜?×?0×?0×?0×?00×(标于物镜筒,如“Plan 40×/0.65”表?0倍物镜):/p>
目镜?0×?5×(标于目镜边缘,如“WF10×/18mm”):/p>
中间附件?.5×(摄像适配器)?.5×(分光棱镜)等、/p>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">标准公式:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">典型组件倍率:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">数码成像系统放大倍数修正/p>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">显示器放大倍率:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">总数码放大倍数:
二、分场景计算示例
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">光学观察(常规目视)/td> | 40×物镜 + 10×目镜 + 无中间附仵/td> | 40×10=400× |
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">摄像记录(适配器介入)/td> | 50×物镜 + 0.5×适配 + 48×显示器放?/td> | 50×0.5×48=1200× |
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">分光多通道系统/td> | 20×物镜 + 10×目镜 + 1.5×分光棱镜 | 20×10×1.5=300× |
三、校准与验证方法
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">标准标尺泔/span>:
使用经认证的刻度标尺(如1mm/100格,每格10μm);
在显微镜下观察标尺,调节倍数使显示器/目镜?格对应理论值;
计算误差:若50×物镜下标?格显示为0.2mm,实际应丹span class="katex" style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-alternates: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-stretch: normal; font-size: 1.21em; line-height: 1.2; font-family: KaTeX_Main, "Times New Roman", serif; text-rendering: auto;">
,则系统误差?0%,需检查中间附件倍率、/p>span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">分辨率板验证:
使用USAF 1951分辨率板,根据可分辨的最小条纹间距()计算有效放大倍数9span class="katex-display" style="margin: 1em 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; display: block; overflow: auto hidden; text-align: center;">
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">限制条件:总放大倍数不应超过物镜空放大阈值(),例:NA=0.65的物镜,最大有效倍数?50×、/p>
四、特殊情形处琅/h4>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">连续变倍系绞/span>:
体视显微镜变倍范?.67×-4.5×,需读取刻度环实时倍率值参与计算、/p>
例:主物?× + 变倍环3× + 10×目镜 总倍数=2×3×10=60×、/p>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">轴向放大率修止/span>:
3D成像时,轴向放大玆span class="katex" style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-alternates: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-stretch: normal; font-size: 1.21em; line-height: 1.2; font-family: KaTeX_Main, "Times New Roman", serif; text-rendering: auto;">
,需单独标注、/p>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">连续变倍系绞/span>:
体视显微镜变倍范?.67×-4.5×,需读取刻度环实时倍率值参与计算、/p>
例:主物?× + 变倍环3× + 10×目镜 总倍数=2×3×10=60×、/p>
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">轴向放大率修止/span>:
3D成像时,轴向放大玆span class="katex" style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-alternates: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-stretch: normal; font-size: 1.21em; line-height: 1.2; font-family: KaTeX_Main, "Times New Roman", serif; text-rendering: auto;">
,需单独标注、/p>
五、常见错误与修正
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">忽略中间附件倍率/td> | 未计?.5×摄像适配?/td> | 总倍数=物镜×目镜×适配?/td> |
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">混淆数码/光学放大/td> | 将数码缩放(?00%软件放大)视为光学放?/td> | 光学与数码放大需分开标注 |
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">空放大超陏/span>/td> | 100×物镜(NA=1.3)搭?0×目镜 3000×(远?000×NA(/td> | 更换低倍目镜或高NA物镜 |
六、放大倍数与分辨率关系
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">理论依据:
物镜分辨玆span class="katex" style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; font-variant-numeric: normal; font-variant-east-asian: normal; font-variant-alternates: normal; font-kerning: auto; font-optical-sizing: auto; font-feature-settings: normal; font-variation-settings: normal; font-stretch: normal; font-size: 1.21em; line-height: 1.2; font-family: KaTeX_Main, "Times New Roman", serif; text-rendering: auto;"> );
(κ550nm时,有效总放大倍数应满足:
span style="margin: 0px; padding: 0px; -webkit-font-smoothing: antialiased; scrollbar-color: rgba(184, 186, 193, 0.6) transparent; scrollbar-width: thin; -webkit-text-stroke: var(--cos-font-medium-stroke); font-weight: 600 !important;">应用实例:
物镜40×/0.65,理论分辨率 :/p>
有效放大倍数范围?25×~650×:/p>
若搭?5×目镜,总倍数=40×15=600×(符合有效范围)、/p>
通过精确计算与校准,可确保金相分析中显微组织的真实尺度表征。建议每次更换光学元件后,用标准标尺重新验证放大倍数,避免测量误差、/p>
