鐩殑:鐮擖span style="background: rgb(255, 255, 255); line-height: 150%; font-family: 瀹嬩綋;">绌迭/span>娴嬪畾铏庢潠涓ぇ榛勭礌鐨勫惈閲忥紱铏庢潠灞炰簬钃肩铏庢潠灞炴鐗╋紝骞茬嚗鐨勬牴鍏锋湁绁涢鍒╂箍鏁ｇ榾瀹氱棝姝㈠挸鍖栫棸鍔熸晥锛屾槸浼犵粺涓嵂锛屽叾鍖栧鎴愬垎澶嶆潅锛屾墍鍚湁鐨勪富瑕佹垚鍒嗗ぇ榛勭礌鍏锋湁鎶楄偪鐦ゆ姉鐥呮瘨鎶楄弻鎶楃値骞冲枠绛変綔鐢ㄣ€侟/span>

鏂规硶:閲囩敤鍙屾尝闀胯杽灞傛壂鎻忔硶锛屾尝闀挎壂鎻忔潯浠讹細纭呰兌G钖勫眰鏉匡紝浠ョ敳鑻?閱嬮吀涔欓叝-鐢查吀锛?5:2:1锛変负灞曞紑鍓傦紝鍙屾尝闀垮弽灏勬硶閿娇鎵弿锛孅/span>位s=440nm锛孅/span>位r=660nm锛岀嚎鎬у弬鏁颁负3锛屽厜鏉熺嫮缂濓細2mm脳2mm

缁撴灉:鐐规牱閲?.202ug-1.010ug,绾挎€у叧绯昏壇濂斤紝Y=1424.926X+440.17,r=0.9992;骞冲潎鍥炴敹鐜囦负98.04%锛孅/span>RSD=1.47%(n=6)

缁撹:鏂规硶绠€渚垮噯纭紝鍙敤浜庢湰鍝佽川閲忔帶鍒迭/span>

1鏉愭枡涓庝华鍣?/span>

1.1鏉愭枡

1.11铏庢潠鍘熻嵂鏉忺/span>:钃肩铏庢潠灞炴鐗╃殑骞茬嚗鏍瑰拰鏍硅寧

1.12璇曞墏:瀵圭収鍝?/span>:澶ч粍绱犲鐓у搧锛屽叾浣欒瘯鍓傚潎涓哄垎鏋愮函

1.2浠櫒Biostep- CD60钖勫眰鎵弿浠強鍒嗘瀽杞欢;楂樻晥纭呰兌G钖勫眰鏉匡紝鐢靛瓙澶╁钩锛孅/span>5ul寰噺杩涙牱鍣紝

2鏂规硶

2.1瀹為獙鏉′欢

2.1.1灞傛瀽锛氳杽灞傛澘锛氱鑳迭/span>G棰勫埗鏉匡紙10cm脳20cm锛堻/span>,涓寸敤鍓岦/span>110鈩冩椿鍖朁/span>30min

灞曞紑锛氱敳鑻?/span>-閱嬮吀涔欓叝-鐢查吀锛圏/span>15:2:1锛堻/span>,楗卞拰15min鍚庡睍寮€锛屽彇鍑烘櫨骞茶嚜鐒跺厜涓嬫瑙嗕负榛勮壊鏂戠偣

2.1.2鎵弿鏉′欢鍙屾尝闀挎壂鎻忔硶閿娇鎵弿锛屾祴瀹氭尝闀军/span>位s=440nm,鍙傛瘮娉㈤暱位r=600nm,绾挎€у弬鏁颁负Sx=3锛屽厜鏉熺嫮缂濓細2mm脳2mm

2.2瀵圭収鍝佹憾娑插埗澶囷細鍙栧ぇ榛勭礌瀵圭収鍝佺害5.05mg锛岀簿瀵嗙О瀹氾紝缃?/span>50ml瀹归噺鐡朵腑锛屽姞鐢查唶婧惰В绋€閲婅嚦鍒诲害锛屾憞鍖€锛屼綔涓哄鐓у搧婧舵恫銆侟/span>

2.2.2渚涜瘯鍝佹憾娑茬殑鍒跺

鍙栬檸鏉栫矇纰庤繃60鐩瓫锛岀О鍙栫害0.35g锛岀簿瀵嗙О瀹氾紝鍔犺捀棣忔按30ml鍙婄洂閰伏/span>3ml锛屽姞鐑洖娴?/span>2h锛屾斁鍐凤紱鍔犳隘浠垮洖娴佹彁鍙朁/span>2娆★紝姣忔鍔犳隘浠军/span>50ml锛屽洖娴?/span>1h锛屾斁鍐凤紝鍒嗗彇姘豢灞傦紱鍚堝苟锛氶吀姘村眰缃垎娑叉紡鏂椾腑锛岀敤姘豢鎻愬彇涓夋锛屾瘡娆狘/span>30ml锛屽悎骞舵隘浠挎彁鍙栨恫锛岄€氳繃瑁呮湁鏃犳按纭吀閽犵殑婕忔枟锛屾护鍣ㄧ敤灏戦噺姘豢娲楁钉锛屾礂娑插苟鍏ユ护娑蹭腑锛屽洖鏀舵隘浠匡紝娈嬫福鍔犲皯閲忕敳閱囨憾瑙ｏ紝骞剁敤鐢查唶瀹氬浜嶞/span>10ml瀹归噺鐡朵腑锛屾憞鍖€锛屼綔涓轰緵璇曞搧婧舵恫銆侟/span>

2.3鏂规硶瀛︾爺绌迭/span>

2.3.1绾挎€у叧绯狐/span>:绮惧瘑鍚稿彇瀵圭収鍝佹憾娑秉/span>2,4,6,8,10ul,鐐逛簬鍚屼竴纭呰兌G钖勫眰鏉夸笂锛屼緷涓婅堪鏉′欢灞曞紑鍚庤繘琛屾壂鎻忔祴瀹氥€備互鍏跺嘲闈㈢Н绉垎鍊间负绾靛潗鏍囷紝浠ョ偣鏍烽噺涓烘í鍧愭爣锛屼綔鍥撅紝寰椾竴鏉＄洿绾匡紝鍥炲綊鏂圭▼锛欬/span>Y=1424.926X+ 440.17锛孅/span>r=0.9992.缁撴灉琛ㄦ槑锛屽ぇ榛勭礌鍦?/span>0.202-1.010ug鑼冨洿鍛堢幇鑹ソ鐨勭嚎鎬у叧绯汇€傜粨鏋滆琛?/span>1

琛?/span>1銆€绾挎€у叧绯昏€冨療

2.3.2銆€绋冲畾鎬ц瘯楠孅/span>鍙栨牱鍝?/span>2ul鐐逛簬钖勫眰鏉夸笂锛屽睍寮€銆傛瘡闂撮殧15min娴嬪畾涓€娆★紝1h鍐呮祴瀹欬/span>5娆★紝绉垎鍊煎垎鍒负1547.28,1515.13,1508.27,1468.45,1513.32锛孅/span>RSD=1.66%,缁撴灉琛ㄦ槑1h鍐呯ǔ瀹欬/span>

2.3.3绮惧瘑搴﹀疄楠孅/span>鍙栧鐓у搧6ul鐐逛簬鍚屼竴钖勫眰鏉夸笂锛屽睍寮€锛屽皢鍚屼竴鏉夸笂鐨凕/span>5涓偣鏍烽噺鐩稿悓鐨勭偣鎵弿锛岃褰曞嘲闈㈢Н锛岀Н鍒嗗€煎垎鍒负1328.97,1316.54,1320.17,1332.54,1310.27锛孅/span>RSD=0.69%,缁撴灉琛ㄦ槑锛岀簿瀵嗗害鑹ソ銆侟/span>

2.3.4閲嶅鎬у疄楠孅/span>鍙栬檸鏉栫矇鏈?/span>5浠斤紝姣忎唤绾?/span>0.37g锛岀簿瀵嗙О瀹氾紝鎸夆€滄牱鍝佹憾娑茬殑鍒跺鈥濋」涓嬪鐞嗭紝鍙朁/span>2ul鐐逛簬钖勫眰鏉夸笂骞舵祴瀹氾紝缁撴灉瑙佽〃2锛岀粨鏋滆鎵规牱鍝佸惈澶ч粍绱燑/span>1.05%锛孅/span>RSD=1.73%,璇存槑閲嶅鎬ц壇濂姐€侟/span>

琛?/span>2閲嶅鎬у疄楠孅/span>

2.3.5鍔犳牱鍥炴敹鐜囪瘯楠孅/span>绮惧瘑绉板彇宸茬煡鍚噺鐨勬牱鍝佸叡6浠斤紝鍚勫姞鍏ュ埌澶ч粍绱犲鐓у搧閲廃/span>3.0-4.5mg锛屾寜鐓р€滀緵璇曞搧娑茬殑鍒跺鈥濋」涓嬪埗澶囦緷娉曟祴瀹氬悇璇曢獙娑蹭腑澶ч粍绱犵殑鍚噺锛岀粨鏋滆琛?/span>3.缁撴灉骞冲潎鍥炴敹鐜囦负98.04%锛孅/span>RSD=1.47%

琛?/span>3鍔犳牱鍥炴敹鐜囪瘯楠孅/span>