白金会员
已认?/p>
胱硫醚是同半胱氨酸和胱氨酸的结合物，在哺乳动物体内做为含硫氨基酸的代谢中间体，参与半胱氨酸和蛋氨酸的相互转化、/span>
1仪器及试剁/span>
1.1Biochrom30+氨基酸分析仪，双独立电子陶瓷微量泵，茚三酮柱后衍生系统，4.6´200mm阳离子交换树脂柱、/span>
1.2样品 生理体液标准?/span>
1.3进样体积 20微升
1.4样品 10nmol (胱氨 5nmol)
2?/span>标样配置
氨基酸混合标准储备液：含有天冬氨酸、苏氨酸筈/span>43种常见生理体液氨基酸
行号 |
氨基酷/span> |
行号 |
氨基酷/span> |
||
1 |
Pser |
磷酸丝氨酷/span> |
2 |
Taur |
牛磺酷/span> |
3 |
Pea |
磷酸乙醇胹/span> |
4 |
Urea |
尿素 |
5 |
Asp |
天冬氨酸 |
6 |
Hypro |
羟脯氨酸 |
7 |
Thr |
苏氨酷/span> |
8 |
Ser |
丝氨酷/span> |
9 |
Asn |
天冬酰胺 |
10 |
Glu |
谷氨酷/span> |
11 |
Gln |
谷氨酰胺 |
12 |
Sarc |
肌氨酷/span> |
13 |
AAAA |
a-氨基己二酷/span> |
14 |
Pro |
脯氨酷/span> |
15 |
Gly |
甘氨酷/span> |
16 |
Ala |
丙氨酷/span> |
17 |
Citr |
瓜氨酷/span> |
18 |
Aaba |
a-氨基丁酸 |
19 |
Val |
缬氨酷/span> |
20 |
Cys |
胱氨酷/span> |
21 |
Met |
蛋氨酷/span> |
22 |
Cysth |
胱硫醙/span> |
23 |
Ile |
异亮氨酸 |
24 |
Leu |
亮氨酷/span> |
25 |
Nleu |
异亮氨酸 |
26 |
Tyr |
酪氨酷/span> |
27 |
Bala |
b-丙氨酷/span> |
28 |
Phe |
苯丙氨酸 |
29 |
Aiba |
b-氨基异丁酷/span> |
30 |
Homocyst |
高半胱氨酷/span> |
31 |
Gaba |
g-氨基丁酸 |
32 |
Ethan |
乙醇胹/span> |
33 |
Amm |
氨气 |
34 |
Hylys |
羟赖氨酸 |
35 |
Orn |
鸟氨酷/span> |
36 |
Lys |
赖氨酷/span> |
37 |
1-mhis |
1-甲基组氨酷/span> |
38 |
His |
组氨酷/span> |
39 |
Trp |
色氨酷/span> |
40 |
3- mhis |
3-甲基组氨酷/span> |
41 |
Ans |
鹅肌肼/span> |
42 |
Car |
肌肽 |
43 |
Arg |
精氨酷/span> |
|||
3分析方法
氨基酸分析仪分析方法参数９/span>
1)色谱柱：Biochrom LI型阳离子交换树脂柱，4.6´200mm
2)分离程序９/span>
标准品的分离程序
NO. |
Time |
Temp |
Buffer |
Pump |
Nin |
Rec |
Commands |
1 |
01:00 |
34 |
1 |
25.0ml/h |
ON |
ON |
|
2 |
00:00 |
34 |
1 |
25.0ml/h |
ON |
ON |
Reset |
3 |
08:00 |
34 |
1 |
25.0ml/h |
ON |
ON |
Load |
4 |
33:00 |
34 |
2 |
25.0ml/h |
ON |
ON |
|
5 |
25:00 |
50 |
2 |
25.0ml/h |
ON |
ON |
|
6 |
60:00 |
20 |
2 |
25.0ml/h |
ON |
ON |
|
7 |
15:00 |
20 |
2 |
25.0ml/h |
ON |
ON |
|
8 |
10:00 |
68 |
3 |
25.0ml/h |
ON |
ON |
|
9 |
29:00 |
68 |
3 |
25.0ml/h |
ON |
ON |
|
10 |
07:00 |
77 |
4 |
25.0ml/h |
ON |
ON |
|
11 |
25:00 |
85 |
5 |
25.0ml/h |
ON |
ON |
|
12 |
12:00 |
85 |
5 |
25.0ml/h |
ON |
ON |
|
13 |
06:00 |
85 |
6 |
25.0ml/h |
ON |
ON |
|
14 |
06:00 |
85 |
1 |
25.0ml/h |
ON |
ON |
|
15 |
30:00 |
34 |
1 |
30.0ml/h |
OFF |
OFF |
|
16 |
05:00 |
34 |
1 |
25.0ml/h |
ON |
OFF |
|
End |
4附图
氨基酸标准品图谱
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