全自动外泌体荧光检测分析系绞�/strong>

应用方向及主要特�?/strong>

生物标记物共定位 可量�?种标记物的表达情冴�/p>	计数分析 直接从样品中计算抗原阳性外泌体的数量，无需提纯
粒径分析 高精度统计外泌体的颗粒大小及分布	检测外泌体内容�?/strong> 使用ExoView Cargo试剂盒可探测外泌体内部核酸的装载情况，分析装载率
荧光检浊�/strong> 具备3个荧光通道，能够探测单个蛋白的结合	线性工作流稊�/strong> 9个样品的全自动分枏�/p>
无须纯化 无需担心纯化带来的误差，更精确的测量样品间的表征和表达信息的差异	多重样本分析 可使�?种表面标记物来筛选外泌体

粒径分析

全自动外泌体荧光检测分析系绞�/strong>能够寸�50 nm的外泌体进行全方面的表征，无论是粒径尺寸、粒径分布还是外泌体的亚型均可在一次测试中得到。并且所用来测试的样本无需进行纯化，避免因纯化带来的样本偏差、�/span>

分析不同大小和不同尺度的外泌体亚群，在CD171过表达系统中�?00 nm以上的外泌体仅表达了CD171、�/span>

量化外泌体亚羣�/strong>

通过抗体捕获的模式，全自动外泌体荧光检测分析系绞�/strong>能够量化含有不同标记物的外泌体亚群，并对不同种群的外泌体进行特异性计数和分析。整个过程无需纯化，并且线性范围可跨越3个数量级、�/span>

TSPAN8阳性细胞外囊泡的稀释曲线。使�?:3比例的梯度稀释。用R²= 0.9986计算与TSPAN8荧光数据的线性拟合、�/span>

检测外囊泡中的装载�?/strong>

MISEV建议在表征外泌体和外囊泡时，应当同时测量表面和载体蛋白。使用ExoView芯片可穿透外泌体，探测膜内蛋白和载体。并且单次可定量3种表面、管腔蛋白、�/span>

检测细胞外泌体内的Syntenin、�/span>

WT细胞在未穿膜的条件下几乎观测不到Syntenin的信号。进行穿膜处理后可以观测到Syntenin信号，而KO之后信号消失

生物标志物共定位

全自动外泌体荧光检测分析系绞�/strong>能够在单个外泌体样本上检测多�?种标记物，并同时提供外泌体的其它表征数据诸如计数、颗粒尺寸统计等、�/span>

无需纯化

全自动外泌体荧光检测分析系绞�/strong>只测量含有靶标抗原的特定细胞外泌体群体。仅需35ul的稀释样品，即可直接获得外泌体的表型、l粒径和计数信息。并且无需担心污染物对测试的影响、�/span>

无论纯化与否，Exoview均可进行分析

ExoView? Kit 配置清单:

ExoView? Tetraspanin Kits	CD9 - 捕获抖�/span>佒�/span> CD63 - 捕获抗体 CD81 - 捕获抗体 Mouse IgG 阴性对� CD9、CD63、CD81三色共标记荧光抗体、�/span>
ExoView? Tetraspanin Plasma Kits	CD9 - 捕获抗体 CD63 - 捕获抗体 CD81 - 捕获抗体 CD41a - 捕获抗体 多达三种定制化捕获抗佒�/span> 同种阴性对� CD9、CD63、CD81三色共标记荧光抗体、�/span>
ExoView? Tetraspanin Custom Kits	CD9 - 捕获抗体 CD63 - 捕获抗体 CD81 - 捕获抗体 多达三种定制化捕获抗佒�/span> 同种阴性对�?/span> CD9、CD63、CD81三色共标记荧光抗体、�/span>
ExoView? Cargo Kits	兼容所有ExoView Kit 标准 CD9� CD63和CD81 捕获抗体 Mouse IgG 阴性对� ExoView Cargo 试剂 CD9、CD63、CD81三色共标记荧光抗体、�/span> Sytenin荧光抗体

发表文章

2020年已发表文献

1. Cytokine profiling in serum-derived exosomes isolated by different methods� Jung HH� Kim JY� Lim JE� Im YH�Nature Scientific Reports2020

2. Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy� Cho K� Kook H� Kang S Lee J�JEV2020

3. Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis� Rogers MA� Buffolo F� Schlotter F� Atkins SK� Lee LH� Halu A� Blaser MC� Tsolaki E Higashi H� Luther K� Daaboul G� Bouten CVC� Body SC� Singh SA� Bertazzo S� Libby P� Aikawa M� Aikawa E�Cell Biology2020

4. Targeting tumor-derived exosomes using a lectin affinity hemofiltration device� Marleau AM� Jacobs MT� Gruber N� Rodell TC� Ferrone S� Whiteside TL�Cancer Research2020

5. Extracellular Vesicle and Particle Biomarkers DefineMultiple Human Cancers. Hoshino A� Kim HS� Bojmar L� Gyan KE� Cioffi M� Hernandez J� Zambirinis CP� Rodriques G� Molina H� Heissel S� Mark MT� Steiner L� Benito Martin A� Lucotti S� Di Giannatale A� Offer K� Nakajima M� Williams C� Lyden D.�Cell2020

6. Reporter mice for isolating and auditing cell type‐specific extracellular vesicles in vivo� McCann JV� Bischoff SR� Zhang Y� Cowley DO� Sanchez-Gonzalez V� Daaboul GG� Dudley AC�Genesis2020

7. High yield and scalable EV production from suspension cells triggered by turbulence in a bioreactor� Grainger A� Wilhelm C� Gazeuah F� Silva A�Cytotherapy2020

8. Extracellular Vesicles: A New Frontier for Research in Acute Respiratory Distress Syndrome� Mahida RY� Matsumoto S� Matthay MA.American Journal of Respiratory Cell and Molecular Biology2020

9. Small extracellular vesicles modulated by ��V��3 integrin induce neuroendocrine differentiation in recipient cancer cells� Quaglia F� Krishn SR� Daaboul GG� Sarker S� Pippa R� Domingo-Domenech J� Kumar G� Fortina P� McCue P� Kelly WK� Beltran H� Liu Q� Languino LR.�Journal of Extracellular Vesicles� Volume 9� Issue 1� 2020

10. Characterisation of extracellular vesicles surface markers and co-expression studies with single particle interferometric imaging platform� Kusuma G� Lim R.�Cytotherapy� Volume 22� Issue 5� 2020

11. Engineering mesenchymal stem cell paracrine activity with 3D culture� Kusuma G� LiA� Zhu D� McDonald H� Chmabers D� Frith J� Lim R.＋�strong>Cytotherapy� Volume 22� Issue 5� 2020

12. Release of extracellular vesicle miR-494-3p by ARPE-19 cells with impaired mitochondria，Ahn JY� Datta S� Cano M� Mallick E� Rai U� Powell B� Tian J� Witwer KW� Handa JT� Paulaitis ME.＋�strong>Biochim Biophys Acta Gen Subj. 2020Mar 30:129598. (doi: 10.1016/j.bbagen.2020.129598)

13. Advantageous Antibody Microarray Fabrication Through DNA-Directed Immobilization: A Step Toward Use of Extracellular Vesicles in Diagnostics� Brambila D� Sola L� Chiari M.�Talanta 2020

15. Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis� Gori A� Romanato A� Bergamaschi G� Strada A� Gagni P� Frigerio R� Brambilla D� Vago R� Galbiati S� PIcciolini S� Bedoni M� Daaboul G� Chiari M� Cretich M.�Journal of Extracellular Vesicles�Volume 9� Issue 1� 2020

16. Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation� Crescitelli R� L?sser C� Jang SC� Cvjetkovic A� Malmh?ll C� Karimi N� H??g JL� Johansson I� Fuchs J� Thorsell A� Gho YS� Bagge RO� L?tvall J�Journal of Extracellular Vesicles� 2020

17. Extracellular Vesicles Derived from Induced Pluripotent Stem Cells Promote Renoprotection in Acute Kidney Injury Model� Collino F� Lopes JA� Tapparo M� Tortelote GG� Kasai-Brunswick TH� Lopes GMC� Almeida DB� Skovronova R� Wendt CHC� de Miranda K� Bussolati B� Vieyra A� Soares Lindoso R.�Cells� 2020

18. Extracellular Vesicles: A New Frontier for Research in Acute Respiratory Distress Syndrome，Mahida RY� Matsumoto S� Matthay MA.American Journal of Respiratory Cell and Molecular Biology� 2020

19. Exosomes A clinical COmpendium - Methods for exosome isolation and characterization，Zhou M� Weber SR� Zhao Y� Chan H� Sundstrom JM.Exosomes A Clinical Compendium 2020

20. Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence，Panagopoulou MS� Wark AW� Birch DJS� Gregory CD.Journal of Extracellular Vesicles� 2020

21. Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle Yan W� Jiang S�Trends in Cancer 2020

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如何使用全自动外泌体荧光检测分析系绞�/strong>ExoView进行外泌体测量？