仪器简介：

Rapid-quench kinetic analysis has become the method of choice for examination ofenzyme reaction pathways. The KinTek quench-flow enables the most definitive experiments to be performed most efficiently� preserving precious biological samples and providing accurate� reliable data. The KinTek RQF-4 Rapid Quench-Flow Instrument has become the internationalstandard for analysis of enzyme reaction mechanisms. The unique features of the RQF-4 enable unprecedented studies onDNA polymerase mechanismswhere the need for performing single turnover experiments is especially significant and minimal volumes are needed to conserve the expensive synthetic oligonucleotide substrates.

技术参数：

Computer-controlled servo motor drive system for smooth� reliable performance.

Kinetics response time:Shortest reaction time 7 msec.

Minimum Sample Volume:15 - 20 microliters per sample per shot.

Maximum Sample Volume:5 ml per sample per shot.

Syringe Volumes:5 ml standard. Syringes of 0.25� 0.5� 1.0� 2.0 and 10.0 ml are also available.

Temperature Range:4 - 70��C range of temperature is maintained by circulating water bath (not provided).

主要特点９�/strong>

The KinTek RQF-4 uses acomputer-controlledservo motor drive� which provides precise and reproducible setting of reaction times. A major advance over stepper motor technology� the high torque� low inertia� brushless servo motor and high performance� digital power supply provides essentially instantaneous starts and stops of the drive syringes.No other quench-flow provides such performance.

Coupled to a unique� precision valve system� the KinTek RQF-4 provides unparalleled ease of use and reliability.

Four-syringeexperiments involving three mixing events and two programmed reaction times (Double Delay Quench) can be achieved using the computer-controlled motor drive.