BTX ECM830方波电穿孔系�?参数-价格-中国粉体罐�/title>

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美国BTX公司�?983年成立以来，以电穿孔仪、电融合仪为主要产品，是**家生产高质量电穿孔仪器的生产厂家，开创了许多在电穿孔、电融合、转染、转化等方面�?*产品和技术，不仅为全球在此领域的研究者提供了BTX专业产品，而且拥有多至25种的配件，包括：电穿孔、电融合、活体导入配件等等，成为全球**专业的转基因、电融合、活体转基因仪及配件的生产厂家、�/p><p> ECM? 830是为体外和体内电穿孔设计的方形波电穿孔系统，方波技术为研究者提供了更高的细胞转染率和存活率。ECM830可与BTX的各种专业电极及配件结合使用，应用范围十分广泛，如将基因、药物和蛋白质导入活体体�?体外，核移植、胚胎操作、植物原生质体以及细菌和酵母等等、�/p><p></p><p><span><strong>可靠� </strong></span></p><p><span>温和的方波提高了电穿孔后的细胞存活率，从而保证了转化或转染效玆�/span></p><p><span><strong>可操作�?/strong></span></p><p><span>宽的电压和脉冲持续时间范围，脉冲参数可精确设定，满足各种应用目的和实验方案的参数设定、�/span></p><p><span><strong>监控显示</strong></span></p><p><span>液晶屏幕的高分辨率显示电压峰值和脉冲时间，脉冲数，脉冲间隔，使参数的优化、故障的排除和记录成为可能、�/span></p><p><span><strong>安全性能</strong></span></p><p><span>电弧淬灭功能，使由电弧引起的损害降至*低；短路保护，避免脉冲发生器遇到短路时被损坏、�/span></p><p><span><strong>兼容�?/strong></span></p><p><span>BTX各种电穿孔或电融合电极；BTX MOS 多孔板电穿孔系统；VIP 3000监测系统；脚控开关、�/span></p><table cellspacing="0" cellpadding="2" border="1" bordercolor="#f2f2f2" width="100%"><tbody><tr><td colspan="3"><p><strong><span>技术参�?/span></strong></p></td></tr><tr><td rowspan="4"><p><span>高压模式</span></p></td><td><p><span>电压</span></p></td><td><p><span>50-3000V</span><span>＋�/span><span>5V</span><span>调进</span></p></td></tr><tr><td><p><span>电阻</span></p></td><td><p><span>56 ��</span></p></td></tr><tr><td><p><span>电容</span></p></td><td><p><span>111��F</span></p></td></tr><tr><td><p><span>波长</span></p></td><td><p><span>10-600��sec</span><span>＋�/span><span> 1��sec</span><span>分辨玆�/span></p></td></tr><tr><td rowspan="4"><p><span>低压模式</span></p></td><td><p><span>电压</span></p></td><td><p><span>5-500V</span><span>＋�/span><span>1V</span><span>调进</span></p></td></tr><tr><td><p><span>电阻</span></p></td><td><p><span>56 ��</span></p></td></tr><tr><td><p><span>电容</span></p></td><td><p><span>4000��F</span></p></td></tr><tr><td><p><span>波长</span></p></td><td><p><span>10-999��sec/1��sec</span><span>分辨玆�/span></p><p><span>1-999msec/1 msec</span><span>分辨玆�/span></p><p><span>1-10sec/0.1 sec</span><span>分辨玆�/span><strong></strong></p></td></tr><tr><td colspan="2"><p><span>脉冲�?/span></p></td><td><p><span>1-99</span><span>�?/span></p></td></tr><tr><td colspan="2"><p><span>脉冲间隔</span></p></td><td><p><span>100msec</span><span>，�/span><span>10sec</span></p></td></tr></tbody></table><p><span>多种专业融合电极、活体电极可供选择，具体请看其介绍，或查看如下文件�?/span></p><p><span>BTX融合电极&活体电极</span></p><p><strong><span>一：仪器设置：</span></strong><span>（旋转参数按制键钮）</span><strong></strong></span></p><p><span>1 mode: </span><span>电压模式不可谂�/span></p><p><span>2 voltage: 5-500V</span><span>＇�/span><span>LV</span><span>低电压模式）可以�?/span><span>1V</span><span>为步进调敳�/span></p><p><span>30-3000V</span><span>＇�/span><span>HV</span><span>高电压模式）可以�?/span><span>5V</span><span>为步进调敳�/span></p><p><span>------</span><span>决定电传孔时细胞孔径的大小（电压释放精度�?/span><span>0.5%� </span><span>电压显示精度�?/span><span>3%</span><span>（�/span></p><p><span>3 PULSE length: 10 � 999us</span><span>＇�/span><span>LV</span><span>低电压模式）可以�?/span><span>1us</span><span>为步进调敳�/span></p><p><span>1-999ms</span><span>＇�/span><span>LV</span><span>低电压模式）可以�?/span><span>1ms</span><span>为步进调敳�/span></p><p><span>1-10s</span><span>＇�/span><span>LV</span><span>低电压模式）可以�?/span><span>0.1s</span><span>为步进调敳�/span></p><p><span>10us-600us</span><span>＇�/span><span>HV</span><span>高电压模式）可以�?/span><span>1us</span><span>为步进调敳�/span></p><p><span>-----</span><span>维持细胞孔径张开的时闳�/span></p><p><span>4 PULSE number:1-99</span></p><p><span>5 interval: 100-999ms</span><span>可以�?/span><span>1ms</span><span>为进制调整：</span><span>1.0-10.0s</span><span>可以�?/span><span>0.1s</span><span>为进制调敳�/span></p><p><span>------</span><span>细胞多次电击脉冲之间的恢复时闳�/span></p><p><span>6 polity :</span><span>不可设置</span></p><p><span>7</span><span>� � 性：抗短路保护设讠�/span></p><p><span>8</span><span>� � 量：</span><span>4000 </span><span>��</span><span>F</span></p><p><span>9</span><span>� � 量： </span><span>10</span><span>��</span><span>s 500A</span></p><p><strong><span>二：安全�?/span></strong></p><p><span>1</span><span>在工作场所提供合适的电源座，位置**远离有水环境，忌在爆炸物或在可传导的污染环境里工作、�/span></p><p><span>2 </span><span>由于仪器是热启动装置，在夏天开机后需预热</span><span>20min</span><span>以蒸发内部水蒸气，且在每次使用完毕后</span><strong><span>将电源开关及电源线拔掉以确保安全、�/span></strong></span></p><p><span>3</span><span>一个人操作仪器以避免不协同对仪器造成损坏、�/span></p><p><span>4 </span><span>操作仪器前，将样品完全准备之后再连接线路以及开机、�/span></p><p><span>5 </span><span>操作池盖合上才可以操作，忌轻易触摸内部金属片、�/span></p><p><strong><span>三：试验操作安全�?/span></strong></p><p><span>实验操作过程中不可避免会出现</span><strong><span>击穿</span></strong><span>现象，会对仪器造成很大损伤，原因有很多。为较好地维护仪器，每次实验时可先用空样品（只有缓冲液）进行试打，以检验仪器安全性和所用缓冲液的纯度，如发现有电穿情况要立刻停止操作分析原因在进行试验、�/span></p><p><span>电爆现象：初始设置参数与反馈参数不一致，反馈值都会很大幅度的降低、�/span></p><p><span>发生电穿的可能原因：</span></p><p><span>１设置参数出现错误：</span><strong><span>场强与电压的关系，公司提供的参考数据是场强需经换算、�/span></strong></span></p><p><span>　<strong><span>Ｅ＝ｖ／ｃ（ｃ为电击杯的间隙（�/span></strong></span></p><p><span>２操作池内部要始终保持干燥、�/span></p><p><span>３如果公司提供的步骤上要求要达到某种冷冻温度和时间一定要按要求进行、�/span></p><p><span>４电击杯和缓冲液**不要有杂质。（主要来自电解质的污染（�/span></p><p><span>５电击杯在使用之前要洗涤干净，使用时间太长则需要及时丢弃、�/span></p><p><strong><span>６两次操作时间间�?*大于０�/span><span>min</span><span>，以确保仪器良好散热、�/span></strong></p><p><strong><span>高电压警告：为了实际操作和安全的原因＋�/span><span>ECM 830</span><span>在施加脉冲的过程中，请不要接触任何电缆或电极连接处，当需要连接或拆下电缆时，要检查系统不在操作中或处于备用状态、�/span></strong></p> </div>  <div class="cxd"> <div class="cxd_tit"><span></span>创新炸�/div> 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