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$(this).css({"color":"#1479d7","border-bottom-color":"#1479d7"}); }); }); </script>  <div class="cpjj"> <span>默片钳是</span><span>研究离子通过膜离子通道运动的一种技�?/span><span>＋�/span><span>即用一微电极封住（钳住）细胞膜片表面，然后测量通过这一部分膜上的电流、�/p><p></span></p><p><strong>基本原理</strong></p><p> 它又称单通道电流记录技术，用特制的玻璃微吸管吸附于细胞表面，使之形�?0�?00的密�?giga-seal)，被孤立的小膜片面积�?#956;m量级，内中仅有少数离子通道。然后对该膜片实行电压钳位，可测量单个离子通道开放产生的pA�?0安培）量级的电流，这种通道开放是一种随机过程。通过观测单个通道开放和关闭的电流变化，可直接得到各种离子通道开放的电流幅值分布、开放几率、开放寿命分布等功能参量，并分析它们与膜电位、离子浓度等之间的关系。还可把吸管吸附的膜片从细胞膜上分离出来，以膜的外侧向外或膜的内侧向外等方式进行实验研究。这种技术对小细胞的电压钳位、改变膜内外溶液成分以及施加药物都很方便、�/p><p></p><p><strong>� 逓�/strong></p><p> 金属离子作用于细胞膜行为的研究细胞膜离子通道的性质鉴定及其:</p><p> 动力学研穵�/p><p> 细胞分泌的研穵�/p><p> 信号转导的研穵�/p><p> 分子生物学研穵�/p><p><strong>应用举例 </strong></p><p> (1). 膜片钳技术在通道研究中的重要作用</p><p> (2).与药物作用有关的心肌离子通道</p><p> (3).对离子通道生理与病理情况下作用机制的研穵�/p><p> (4).对单细胞形态与功能关系的研穵�/p><p> (5).对药物作用机制的研究</p><p> (6). 在心血管药理研究中的应�?/p><p> (7). 创新药物研究与高通量筛逈�/p><p><strong>仪器主要组成及特炸�/strong></p><p> 1、Ti专业荧光显微镜图像分析系统（机型可选）：�/p><p> 2、Axopatch200B/700B电容反馈型膜片钳：�/p><p> 3、数据采集卡软件：�/p><p> 4、灌流系统（8通道，带温度控制）PS25-cell（可选）</p><p> 5、成茂显微操作系统（细胞（�/p><p> 6、电极拉针器：�/p><p> 7、防震台含静电屏蔽网</p><p> 8、监视器</p><p> </div>  <div class="cxd"> <div class="cxd_tit"><span></span>创新炸�/div> <p style=" color: #333; 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