Wes鍏ㄨ嚜鍔ㄨ泲鐧借川鍗拌抗瀹氶噺鍒嗘瀽绯荤粺

銆愭€ц兘鐗圭偣銆慄/span>

浼犵粺铔嬬櫧鍒嗘瀽鐨勫疄楠屾妧鏈富瑕佹槸铔嬬櫧鍗拌抗锛岀敱浜庢搷浣滄楠ゅ锛屾搷浣滅箒鐞愶紝姣忎竴姝ョ殑澶辫閮戒細閫犳垚鍏ㄧ洏澶辫触锛屼粠鑰岄噸澶嶅疄楠岋紝瀵逛簬鐝嶈吹鏍峰搧锛屾洿鏄鍊嶅姞灏忓績銆傚彟澶栵紝璇曢獙娴佺▼闀匡紝缁撴灉閲嶅鎬у樊锛岃杈惧埌濂界殑瀹為獙缁撴灉锛屾垚鏈篃浼氬緢楂樸€傚悓鏃讹紝浼犵粺鐨勮泲鐧藉嵃杩瑰疄楠屾妧鏈棤娉曞疄鐜板噯纭畾閲忥紝鍏呭叾閲忔槸鍗婂畾閲忋€侟/span>

Wes绯荤粺閲囩敤闈╂柊鐨凕/span>Simple Western鎶€鏈紝棰犺30澶氬勾浼犵粺鐨凕/span>Western Blot鏂规硶锛屽疄鐜板湪鍚屼竴鏍规牱鍝佺涓畬鎴愭牱鍝佸垎绂汇€佹崟鑾枫€佸浐瀹氾紝鍏嶇柅妫€娴嬪拰瀹氶噺鍒嗘瀽銆侟/span>

1銆佷笉鐢ㄥ埗鑳跺拰璺戣兌锛屼笉鐢ㄨ浆鑶滐紝鍑忓皯瀹為獙姝ラ锛岄伩鍏嶆牱鍝佹崯澶便€侟/span>

2銆佽嚜鍔ㄨ繘琛屼竴鎶椾簩鎶楀鑲插拰鍖栧鍙戝厜妫€娴嬶紝鑷姩杩涜缁撴灉鍒嗘瀽锛屾湁鏁堟彁楂樻娴嬬粨鏋滅殑绮剧‘鎬у拰閲嶅鎬с€侟/span>

3銆佸崟涓牱鍝佷笂鏍蜂綋绉?/span>3-5ul锛岃妭绾︾弽璐垫牱鍝佸拰鎶椾綋銆侟/span>

4銆佷竴娆¤繍琛屾椂闂滁/span>3涓皬鏃讹紝蹇€熼珮鏁堛€侟/span>

5銆佹娴嬬伒鏁忓害楂橈紝浣庤嚦pg绾с€侟/span>

6銆佸疄楠屽畬鎴愬悗鑷姩缁欏嚭璇﹀敖鐨勫垎鏋愭暟鎹紝鍖呮嫭鐩殑铔嬬櫧澶у皬銆佷俊鍣瘮銆佸嘲闈㈢Н鍜屽惈閲忋€侟/span>

7銆佸彲瀵圭粨鏋滆嚜鍔ㄨ繘琛岀簿纭殑瀹氶噺鍒嗘瀽銆侟/span>

Simple Western绯荤粺浣滀负涓€涓垝鏃朵唬鐨勮泲鐧借川鍒嗘瀽鏂版妧鏈紝鑷粠2011骞村簳鎺ㄥ嚭浠ユ潵锛岃幏寰椾簡骞挎硾濂借瘎鍜岃鍙紝Nature Methods鐜囧厛鎶ラ亾璇ユ妧鏈€傜洰鍓嶅叏鐞冮噸瑕佺殑鐮旂┒鏈烘瀯澶у宸茬粡鎷ユ湁璇ユ妧鏈钩鍙?/span>锛孅/span>濡傜害缈伴湇鏅噾鏂ぇ瀛︺€佸搱浣涘ぇ瀛︺€佹柉鍧︾澶у銆佽€堕瞾澶у銆佸姞宸炲ぇ瀛︽埓缁存柉鍒嗘牎銆佸姞宸炲ぇ瀛?*浜氬摜鍒嗘牎銆佸崕鐩涢】澶у銆?/span>Massachusetts General Hospital銆?/span>Mayo Clinic銆?/span>Southwest Hospital銆佺編鍥键/span>CDC銆?/span>FDA銆傚叾涓?/span>NIH鏈堻/span>5鍙般€?/span>National Cancer Institute鏈堻/span>4鍙帮紝Columbia University鏈堻/span>4鍙般€佺編鍥介粯鍏嬪埗鑽湁12鍙般€侀樋鏂埄搴锋湁7鍙般€佸痉鍥键/span>Charite University鏈堻/span>4鍙般€侟/span>

銆愭妧鏈寚鏍囥€慄/span>

1銆佸姛鑳戒笌搴旂敤锛氬彲鑷姩杩涜鍚勭铔嬬櫧璐ㄦ牱鍝佸垎绂汇€佸厤鐤娴嬨€佸畾鎬у拰瀹氶噺鍒嗘瀽锛屽箍娉涘簲鐢ㄤ簬铔嬬櫧璐ㄦ€ц川閴村畾銆佽泲鐧借川瀹氶噺鍒嗘瀽銆佽泲鐧借川鍔熻兘鐮旂┒銆佽泲鐧借川淇グ鍜屽樊寮傝〃杈剧爺绌躲€佹姉浣撶爺绌剁瓑澶氫釜棰嗗煙锛汓/span>

2銆佸叏鑷姩锛氳泲鐧芥牱鍝佷笂鏍峰悗鏃犻渶浠讳綍浜哄伐鎿嶄綔锛岃嚜鍔ㄥ畬鎴忺/span>Western Blot鍒嗘瀽

3銆佹€昏繍琛屾椂闂达細鈮｜/span>3涓皬鏃迭/span>

4銆佹牱鍝佹€昏泲鐧介噺锛氣墹5ug

5銆佹牱鍝佷笂鏍蜂綋绉細鈮｜/span>5ul

6銆佸姞鏍凤細鏈烘鎵嬭嚜鍔ㄥ畬鎴忺/span>25涓牱鍝佺殑鍔犳牱

7銆佸垎绂绘柟寮忥細鏍规嵁鍒嗗瓙閲忓ぇ灏忓垎绂伙紝鑷姩璇嗗埆铔嬬櫧澶у皬骞跺畬鎴愬垎绂狐/span>

8銆佸垎绂昏繃绋嬶細30绉掗噰闆嗕竴娆℃暟鎹紝瀹炴椂鐩戞祴鍒嗙杩囩▼

9銆佸埗鑳讹細鏃犻渶鍒惰兌杩囩▼锛屽噺灏戞搷浣滅▼搴忥紝閬垮厤姹℃煋

10銆佸浐瀹氭椂闂达細200绉掞紝铔嬬櫧鍒嗙鍚庤嚜鍔ㄥ浐瀹氬埌鏍峰搧绠″涓夵/span>

11銆佽浆鑶滐細鏃犻渶杞啘姝ラ锛屼笉鐢ㄦ媴蹇冭浆鑶滄晥鐜囷紝閬垮厤鏍峰搧鎹熷け锛屾彁楂樿泲鐧借川瀹氶噺缁撴灉鐨勫噯纭巼鍜岄噸澶嶆€?/span>

12銆佹姉浣撳鑲诧細鑷姩瀹屾垚涓€鎶椾簩鎶楀鑲插拰娓呮礂

13銆佹娴嬫柟娉曪細鍖栧鍙戝厜娉旤/span>

14銆佹牱鍝佹暟閲忥細鈮?/span>25涓?/span>

15銆佹牱鍝佺瑙勬牸锛氶暱125px锛屽唴寰凕/span>100um锛屾€讳綋绉?/span>400nl

16銆佽繘鏍蜂綋绉細40nL

17銆佺伒鏁忓害锛欬/span>pg绾?/span>

18銆佸姩鎬佽寖鍥达細3 logs

19銆佺粨鏋滃叿鏈夐珮搴﹂噸澶嶆€э細CV鈮｜/span>10%

20銆佺粨鏋滃垎鏋愶細瀹為獙缁撴潫鍚庯紝鏃犻渶浜哄伐鍒嗘瀽锛岃蒋浠朵細鑷姩缁欏嚭铔嬬櫧鍒嗗瓙閲忓ぇ灏忋€佷俊鍣瘮銆佺櫨鍒嗘瘮鍜屽嘲闈㈢Н

21銆佺粨鏋滃憟鐜板舰寮忥細鍖栧鍙戝厜鎴愬儚缁撴灉锛屽嘲鍨嬪浘缁撴灉鍜屾吵閬撳紡缁撴灉

22銆佸畾閲忓垎鏋愶細杩愯缁撴潫鍚庤嚜鍔ㄧ粰鍑烘爣鍑嗘洸绾匡紝鍙?*瀹氶噺鍒嗘瀽

23銆佽蒋浠跺崌绾э細鍏嶈垂鍗囩骇锛屽彲瀹夎鍦ㄤ换鎰忕數鑴戜笂锛屾病鏈夋嫹璐濇暟闄愬埗

24銆佽蒋浠惰缃細瀹㈡埛鍙畾涔夋牱鍝佸悕绉帮紝璁剧疆妫€娴嬫祦绋嬶紝杞欢鎺у埗鏁翠釜绯荤粺鍏ㄨ嚜鍔ㄨ繍琛岋紝鑷姩瀹屾垚铔嬬櫧璐ㄦ牱鍝佷笂鏍枫€佸垎绂汇€佸浐瀹氥€佷竴鎶楀鑲层€佷簩鎶楀鑲诧紝鍏嶇柅鍖栧鍙戝厜妫€娴嬪拰瀹氶噺鍒嗘瀽