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Milo鍗曠粏鑳炶泲鐧借川琛ㄨ揪瀹氶噺鍒嗘瀽绯荤粺锛屾妧鏈潵婧愯嚜缇庡浗**澶у鍔犲窞澶у浼厠鍒╁垎鏍my E. Herr鏁欐巿瀹為獙瀹わ紝璇ュ疄楠?014骞村師鍒涙€ф妧鏈彂琛ㄥ湪Nature Method锛岄鐩甋ingle cell western blot銆侾roteinSimple鍊熷姪寮哄ぇ鐨勮蒋浠跺強纭欢鐮斿彂瀹炲姏锛屽皢鍏跺紑鍙戜负鍗曠粏鑳炶泲鐧借川琛ㄨ揪瀹氶噺妫€娴嬬郴缁燂紝鐢ㄤ簬缁嗚優寮傝川鎬у強绋€缂虹粏鑳炴牱鏈紙姣斿寰幆鑲跨槫缁嗚優绛夛級鐮旂┒銆侟/span>

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Milo鍗曠粏鑳炶泲鐧借川瀹氶噺琛ㄨ揪鍒嗘瀽绯荤粺锛屾槸**娆惧崟缁嗚優姘村钩锛岃泲鐧借川琛ㄨ揪瀹氶噺鍒嗘瀽绯荤粺銆侻ilo绯荤粺閲囩敤**鐨勫井娴佹帶western blot鑺墖锛岄€氳繃鍗曠粏鑳炲井瀛旇璁★紝閲囬泦鍗曠粏鑳烇紝鐒跺悗鍘熶綅瑁傝В缁嗚優锛岄噴鏀捐泲鐧斤紝杩涜铔嬬櫧璐ㄧ數娉筹紝灏嗕笉鍚屽垎瀛愰噺铔嬬櫧杩涜鍒嗙锛屾彁楂樺厤鐤妫€娴嬬壒寮傛€с€備箣鍚庯紝閲囩敤**鎶€鏈繘琛岃泲鐧借川鍘熶綅鎹曡幏锛屼娇鐢╳estern blot楠岃瘉鎶椾綋鍙婅崸鍏夋爣璁颁簩鎶楃洿鎺ユ潅浜わ紝鎵弿浠繘琛岃姱鐗囨壂鎻忓悗锛孲cout杞欢瀵规壂鎻忕粨鏋滆繘琛屾繁搴﹀畾閲忓垎鏋愩€侟/span>

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Milo鍏锋湁鏋佸叾寮哄ぇ鐨勫姛鑳斤細

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2. 鍗曚釜缁嗚優鍙繘琛屾暟鍗佷釜闈惰泲鐧芥娴婞/span>

3. 浠呴渶浣跨敤western blot楠岃瘉鎶椾綋

4. 鍩轰簬western blot鎶€鏈紝铔嬬櫧璐ㄥ垎绂诲悗妫€娴嬶紝鎻愰珮鍏嶇柅瀛︽娴嬬壒寮傛€?/span>

5. 鍏ㄧ▼妫€娴?-6灏忔椂

6. 閫傜敤浜庯細缁嗚優寮傝川鎬х爺绌跺強绋€缂烘牱鏈娴嬨€侟/span>