单分子检测水平的定量细胞生物学检测仪器、�/span>

受激发射损耗（STED）是一种强大的显微技术，可以观察到空间分辨率低于衍射极限的荧光结构、�span>Alba-STED使用脉冲激发和脉冲耗尽方法＇�span>pSTED）结合数字频域荧光寿命成像（FastFLIM）来记录时间分辨光子，从而提高图像分辨率并分离具有相同激发波长的两个荧光标记物、�/span>

关键特色 � Alba-STED for FLIM/FFS:

?pSTED (Pulsed excitation and pulsed STED)

?FastFLIM for time-resolved pSTED acquisition

?Improved image resolution using the phasor plot

?Dual-label excitation

?Fast image acquisition (dwell time: 0.2 ��s)

?High dynamic range (signal up to 60 million counts/s)

测试实例展示Alba-STED for FLIM/FFS

Confocal (left) vs. pSTED (right) images of the actin labeled with the SiR dye in fixed glia cells� acquired by FastFLIM.

Confocal (left) vs. pSTED (right) images of the actin labeled with the SiR dye in fixed glia cells� acquired by FastFLIM.

Confocal images of 60nm fluorescence beads (left); pSTED images (middle); sharpening the pSTED image using a binary filter based on the phasor plots (right).

Dual labels can be separated using pSTED and FastFLIM. Atto 647N and Atto 655 were used as labels; they both are excited by the 640 nm laser. The two dyes are first separated using the phasor plots� and then assigned with two different false colors (Atto 647N - yellow� Atto 655 - purple) to produce the processed and merged pSTED image of the two labels.

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