鍏充簬浼犵粺QuEChERS鏂规硶
QuEChERS鏂规硶鏄?003骞寸敱缇庡浗鍐滀笟閮ㄧ殑鍖栧瀹禔nastassiades鍜孡ehotay绛夌爺绌跺缓绔嬬殑涓€绉嶈兘鏈夋晥鍒嗙姘存灉銆佽敩鑿滀腑鐥曢噺鍐滄畫鐨勬牱鍝佸墠澶勭悊鎶€鏈€俀uEChERS鏄疩uick銆丒asy銆丆heap銆丒ffective銆丷ugged鍜孲afe鐨勭缉鍐欙紝椤惧悕鎬濅箟锛岃繖绉嶅墠澶勭悊鏂规硶鍏锋湁鎿嶄綔绠€渚裤€佹憾鍓備娇鐢ㄩ噺灏戙€佹薄鏌撳皬绛変紭鍔裤€侟/span>
鈻 鍖栧瀹禔nastassiades楂樺害璇勪环Geno/Grinder
Cole-Parmer锛堝師Spex锛夌殑Geno/Grinder楂橀€氶噺缁勭粐鐮旂（鏈裹/span>涓撲负鍔ㄦ鐗╃粍缁囧揩閫熷寑娴嗙爺鍒躲€備娇鐢℅eno 楂橀€氶噺缁勭粐鐮旂（鏈烘敼鑹疩uEChERS鍓嶅鐞嗘柟娉曪紝涓€閿紡鎿嶄綔锛屽彲璋冮鐜囨渶楂樺彲杈?750rmp锛?-2鍒嗛挓瀹屾垚鏍峰搧鍧囪川鍖栬繃绋嬶紝鍙湁鏁堢畝鍖栨彁鍙栨楠ゅ拰鎻愬彇鏃堕棿銆傜壒娈婄殑鍨傜洿鎸崱涓撳埄鎶€鏈紝鍦ㄥ潎璐ㄧ殑鍚屾椂杩樺彲鐮旂（鏍峰搧锛屼娇鎻愬彇鍓傚厖鍒嗕笌鏍峰搧涓殑鍐滆嵂娈嬬暀缁撳悎锛屾娴嬬粨鏋滄洿鎺ヨ繎鐪熷€笺€傝秴楂橀€氶噺鐨凣eno/Grinder楂橀€氶噺缁勭粐鐮旂（鏈烘渶澶氬彲鍚屾椂澶勭悊16涓?0mL鏍峰搧鐡讹紝鏇撮珮鏁堬紝鏍峰搧闂撮噸澶嶆€с€佷竴鑷存€ф洿濂姐€侟/span>
Cole-Parmer锛堝師Spex锛夊叕鍙哥殑搴旂敤宸ョ▼甯堜娇鐢℅eno/Grinder楂橀€氶噺缁勭粐鐮旂（鏈烘敼鑹悗鐨凲uEChERS鏂规硶涓庝紶缁熸柟娉曡繘琛屼簡瀵规瘮瀹為獙锛屽疄楠岀粨鏋滆〃鏄庯紝Geno/Grinder楂橀€氶噺缁勭粐鐮旂（鏈烘敼鑹悗鐨凲uEChERS鏂规硶鍦ㄧ畝鍖栨搷浣滀箣浣欙紝杩樻湁鏁堢殑鎻愬崌浜嗗洖鏀剁巼锛屽疄楠岃繃绋嬪強缁撴灉濡備笅鎵€绀恒€侟/span>
涓轰娇瀹為獙鏍峰搧鏇村叿浠ｈ〃鎬э紝鏈疄楠岄€夌敤鑽夎帗锛堣蒋鎬э級銆佽嫻鏋滐紙瀵嗗害鍜岀‖搴﹀ぇ锛夈€佽姽鑿滐紙瀵屽惈绾ょ淮锛夛紝鍒囨垚6-12mm鐨勫皬娈垫贩鍖€锛岀О鍙?5.1g锛屾斁鍏?0mL绂诲績绠′腑锛堟瘡绉嶆牱鍝佽缃?涓钩琛岋級銆傛瘡涓牱鍝佺摱涓皬蹇冨姞鍏?50渭l鐨勫啘鑽贩鏍囷紙Spex CertiPrep鍏徃璐у彿涓篊AL-CARB-13鏍囧噯婧舵恫锛屾祿搴︼細40渭g/ml锛屽熀搴曪細浜屾隘鐢茬兎锛夛紝鐩栦笂鐩栧瓙锛岀敤鎵嬭交杞绘憞鏅?5s锛屼互纭繚鏁翠釜鏍峰搧鐨勫啘鑽憾娑蹭笌鏍峰搧娣峰寑锛?鈩冨啺绠遍潤缃繃澶溿€侟/span>
浼犵粺QuEChERS鏂规硶
灏?涓蹇冪涓牱鍝佸悎鍦ㄤ竴涓悈鎷屾満涓紝鍧囧寑鍦扮牬纰庢贩鍚堬紝閲嶆柊绉伴噺15.1g杞Щ鑷崇浉鍚岀殑绂诲績绠′腑銆傚姞鍏?g鏃犳按MgSO4銆?.5g鏃犳按閱嬮吀閽犲拰15mL涔欒厛锛堝惈1%鍐伴唻閰革級锛岀洊涓婄洊鍚庢墜鎽?min銆傝崏鑾撶涓憟绮夌孩鑹层€佽嫻鏋滄彁鍙栫墿涓烘贰榛勮壊銆佽姽鑿滄槸楗卞拰缁胯壊銆侟/span>
鑰屽悗3500rmp绂诲績3min锛屽彇涓婃竻娑插垎鎴愪袱绛夊垎锛屽姞鍏?5mL绂诲績绠′腑锛堟瘡涓牱鍝?ppm鏉€铏墏锛夛紝鍔犲叆25mgPSA鍜?mgGCB锛岀洊鐩栧悗鎵嬫憞30s锛?200rmp绂诲績1min鍚庡彇涓婃竻娑层€傜粡杩囨祿缂╁噣鍖栧悗锛屼笂鏈烘娴嬨€侟/span>
Geno/Grinder楂橀€氶噺缁勭粐鐮旂（鏈烘敼鑹疩uEChERS鏂规硶
鍦ㄦ瘡涓牱鍝佺摱涓姞鍏?绮掗櫠鐡风爺纾ㄧ彔锛堣揣鍙?183锛夊拰5mL涔欒厛锛堝惈1%鍐伴唻閰革級锛屾斁鍦℅eno/Grinder楂橀€氶噺缁勭粐鐮旂（鏈轰笂1500rmp鐮旂（2min锛岃崏鑾撴牱鍝佸凡缁忓憟娴嗙姸锛岃嫻鏋滃拰鑺硅彍杈冪‖锛岀爺纾?min鍙互杈惧埌涓€鑷存€у緢濂界殑绯婄姸銆傦紙娣诲姞灏戦噺鐨勬憾鍓傚5mL涔欒厛锛屽彲浠ヨ捣鍒版鼎婊戠殑浣滅敤锛屼娇鐮旂（鏁堟灉鏇翠匠銆傦級鐒跺悗锛屽姞鍏?g鏃犳按MgSO4銆?.5g鏃犳按閱嬮吀閽犮€?0mL涔欒厛锛堝惈1%鍐伴唻閰革級锛岄噸鏂扮洊濂界洊瀛愶紝鏀惧湪Geno 楂橀€氶噺缁勭粐鐮旂（鏈轰笂1500rmp鍧囪川1min銆傚彲浠ョ湅鍒拌崏鑾撶涓殑娑蹭綋鏄矇绾㈣壊鐨勶紝鑻规灉鐨勬槸娣＄传鑹茬殑锛岃姽鑿滄槸楗卞拰鐨勭豢鑹诧紝鎵€鏈夋潗鏂欓兘鏄贩鍚堝潎鍖€銆佸彲娴佸姩鐨勩€侟/span>
鑰屽悗3500rmp绂诲績3min锛屽彇涓婃竻娑插垎鎴愪袱绛夊垎锛屽姞鍏?5mL绂诲績绠′腑锛堟瘡涓牱鍝?ppm鏉€铏墏锛夛紝鍔犲叆25mgPSA鍜?mgGCB锛岀洊鐩栧悗Geno/Grinder缁勭粐鐮旂（鏈轰笂1500rmp鍧囪川30s锛?200rmp绂诲績1min鍚庡彇涓婃竻娑层€傜粡杩囨祿缂╁噣鍖栧悗锛屼笂鏈烘娴嬨€侟/span>
鏋佺畝Geno/Grinder鏀硅壇QuEChERS鏂规硶
涓轰簡绠€鍖朡uEChERS鍓嶅鐞嗘楠わ紝Cole-Parmer锛堝師Spex锛夊簲鐢ㄥ伐绋嬪笀杩樺仛浜嗚繖鏍风殑灏濊瘯锛屽皢6g鏃犳按MgSO4銆?.5g鏃犳按閱嬮吀閽犮€?5mL涔欒厛锛?%鍐伴唻閰革級娣诲姞鍒板惈15.1g鐨勮崏鑾撶蹇冪涓紝鐩栦笂鐩栧瓙鍦℅eno缁勭粐鐮旂（鏈轰笂1500rmp鐮旂（4min锛岀粡杩囨祿缂╁噣鍖栧悗锛屼笂鏈烘娴嬨€傝崏鑾撳緢濂界殑涓庢彁鍙栧墏娣峰悎鍦ㄤ华鍣紝浣嗗湪鑻规灉鍜岃姽鑿滅殑鏍峰搧瀹為獙涓苟涓嶇悊鎯筹紝鐩愬拰閮ㄥ垎鏍峰搧缁撳悎鍦ㄤ竴璧锋椂锛岀爺纾ㄤ粙璐ㄥ鏍峰搧鐨勭爺纾ㄦ晥鏋滃彈闃伙紝鍙疄鐜颁簡閮ㄥ垎纾ㄥ墛銆侟/span>
鏍峰搧鍒嗘瀽
涓ょ鏂规硶娴撶缉鍑€鍖栧悗瀵规牱鍝侀鑹茬殑瀵规瘮锛欬/span>
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鑽夎帗鏍峰搧 |
鑻规灉鏍峰搧 |
鑺硅彍鏍峰搧 |
浼犵粺QuEChERS鏂规硶 |
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Geno鏀硅壇QuEChERS鏂规硶 |
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鈻 鑽夎帗鏍峰搧鐨勬娴嬬粨鏋淈/span>
鈻 鑻规灉鏍峰搧鐨勬娴嬬粨鏋淈/span>
鈻 鑺硅彍鏍峰搧鐨勬娴嬬粨鏋淈/span>
鍙互鏄庢樉鐪嬪嚭锛屼娇鐢℅eno/Grinder楂橀€氶噺缁勭粐鐮旂（鏈哄潎璐ㄥ寲鐨勬牱鍝侊紝妫€娴嬬粨鏋滃潎浼樹簬浼犵粺鏂规硶銆傜櫨鑿屾竻绫荤殑鍐滆嵂浣跨敤Geno/Grinder澶勭悊鐨勬牱鍝佷篃娌℃湁妫€娴嬪埌鍏跺惈閲忥紝浼樹簬鏈瀹為獙涓娇鐢ㄧ殑鏂规硶娌℃湁閽堝鐗瑰畾绫诲瀷鐨勫啘鑽繘琛屼紭鍖栵紝鏈変簺鍐滆嵂涓嶇ǔ瀹氫篃涓嶈冻涓哄銆侟/span>
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缁撹
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