鎽樿

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姒傝堪

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寰瀷鍏夌収鐢熺墿鍙嶅簲鍣紙渭PBR锛夌郴缁燂紝鍙敤浜庣爺绌惰嚜鍏绘垨娣峰悎钀ュ吇鍩瑰吇鏉′欢锛屼絾鐩墠鐨勬蹇电绯诲垪鎶€鏈繕寰堣繙锛岃繘涓€姝ョ殑鏀硅繘鈥斺€斿挨鍏舵槸鍙皟鑺傜収鏄庘€斺€旀槸蹇呰鐨?,9銆侟/p>

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鏂规硶

婊ゅ厜鐗囨ā鍧楀紑鍙慄/strong>

缁忎笓闂ㄨ璁″苟娴嬭瘯鐨勬护鍏夌墖妯″潡锛屽彲鐢ㄤ簬鍦ㄧ嚎鐩戞祴澶氱閲嶈鐨勫厜钀ュ吇鍩瑰吇鍙傛暟锛堢敓鐗╅噺銆佸彾缁跨礌鍚噺鍜宲H鍊硷級銆侟/p>

灏忕悆钘狐/strong>

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鍩瑰吇鍩裹/strong>

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鍦ㄦ憞鐡朵腑棰勫煿鍏狐/strong>

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鍦˙ioLector XT寰瀷鐢熺墿鍙嶅簲鍣ㄤ腑鍩瑰吇

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鍥?. 鍩瑰吇鏈熼棿鐨勭収鏄庡厜璋卞拰杈愮収搴?/p>

灏嗗煿鍏诲弬鏁拌缃负800 rpm銆?5鈩冨拰85% 鐨勭浉瀵规箍搴︼紝骞舵寜10 mL/min娴侀€熷厖鍏ョ┖姘斿拰 2% 鐨凜O2娣峰悎鐗┿€侟/p>

缁撴灉

婊ゅ厜鐗囨ā鍧楁牎鍑咟/strong>

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鐢熺墿閲廃/strong>

缁忎笓闂ㄥ紑鍙戠殑730銆?50鍜?50 nm鐢熺墿閲忔护鍏夌墖妯″潡锛屽彲鍦?.3鈮D750鈮?5鑼冨洿鍐呮樉绀哄嚭鍗撹秺鐨勬牎鍑嗘晥鏋滐紝730 nm妯″潡鏍″噯缁撴灉濡傚浘2.A鎵€绀恒€傛澶栵紝浣跨敤鍙剁豢绱燼鍜屽彾缁跨礌b鏍囧噯鍝侊紝鏈彂鐜板彾缁跨礌骞叉壈銆侟/p>

鍙剁豢绱犲惈閲廃/strong>

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鍙剁豢绱犺崸鍏夊父鐢ㄤ簬娴嬪畾鍏夎惀鍏诲煿鍏荤墿鐨勭敓鐗╅噺14,15銆傚洜姝わ紝浣跨敤鐢熺墿閲忕郴鍒楃█閲婃恫杩涜鏍″噯锛屽簲鐢ㄦ寚鏁伴€掑噺鎷熷悎鏃讹紝鍦ㄤ綆鐢熺墿閲忔祿搴︿笅鍙瀵熷埌鏋佷匠鐨勫垎杈ㄧ巼濡傚浘2.C鎵€绀恒€傛牎鍑嗙粨鏋滆瘉瀹烇紝鍙剁豢绱犺崸鍏夋护鍏夌墖鍣ㄥ彲鍑嗙‘娴嬪畾鐢熺墿閲忔祿搴︼紝鐗瑰埆鏄湪鐢熺墿閲忔祿搴﹁緝浣庣殑鏃跺€欍€侟/p>

pH鍊稽/strong>

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涓虹‘淇濇嫙瀹氭柟娉曞鍏夋紓鐧戒笉鏁忔劅锛屾垜浠湪BioLector XT寰瀷鐢熺墿鍙嶅簲鍣ㄤ腑浣跨敤鏃犳帴绉嶇墿enBBM鍦?00渭mol/m2/s鍏稿瀷鍩瑰吇鏉′欢杩涜鍩瑰吇瀹為獙锛堝浘2.E锛夈€傚疄楠屽紑濮嬪悗锛孋O2娴撳害鐨勫鍔犱娇鍩瑰吇鍩鸿繀閫熼吀鍖栵紙~0.05pH锛夛紝浣嗗湪涔嬪悗鐨?30灏忔椂鍐咃紝娴嬮噺鍊硷紙鍥?.E锛夊缁堜繚鎸佹亽瀹氥€傝繖涓€鐐硅瘉鏄庯紝鍦ㄨ繛缁厜鐓х殑鍩瑰吇鏉′欢涓嬶紝浣跨敤HPTS娴嬪畾pH锛屽彲杩炵画澶氭棩淇濇寔鍑嗙‘銆佺ǔ瀹氱殑娴嬮噺姘村钩銆侟/p>

鍦˙ioLector XT寰瀷鐢熺墿鍙嶅簲鍣ㄤ腑骞惰鍏夎惀鍏诲煿鍏狐/strong>

缁忚繃瀵规护鍏夌墖妯″潡杩涜楠岃瘉涔嬪悗锛屾垜浠紑濮嬮暱鏈熷煿鍏伙紝骞朵娇鐢ㄥ洓涓护鍏夌墖妯″潡杩涜鍦ㄧ嚎鐩戞祴锛?30nm鐨勬暎灏勫厜妯″潡锛屽彾缁跨礌鑽у厜婊ゅ厜鐗囨ā鍧楋紙位ex=450nm锛浳籩m=700nm锛夊拰涓ょHPTS婊ゅ厜鐗囨ā鍧楄繘琛屾瘮鍊紁H娴嬪畾(鍥?)

鍥?. 鎼浇鍏夌収妯″潡鐨凚ioLector XT寰瀷鐢熺墿鍙嶅簲鍣紝涓烘湡16澶╃殑鍩瑰吇涓墍鐩戞祴鐨勬暎灏勫厜銆佸彾缁跨礌鍜宲H鍊稽/p>

濡傚浘3鎵€绀猴紝涓烘湡16澶╃殑鍩瑰吇鐩戞祴涓紝鐢熺墿閲忔祿搴︽寔缁鍔狅紝鐩磋嚦CO2渚涘簲琚叧闂€傚浘涓彲瑙傚療鍒颁簲涓笉鍚岀殑鐢熼暱闃舵锛欬/p>

棣栧厛鏄粸鍚庣殑鎸囨暟鐢熼暱鏈燂紙i.锛夛紝涔嬪悗鏄笁涓笉鍚岀殑绾挎€х敓闀挎湡锛圛I.鑷矷V.锛夛紝鏈€鍚庢槸CO2鑰楀敖鍚庤繘鍏ョ殑姝讳骸闃舵锛圴.锛夈€俻H鍜屽彾缁跨礌淇″彿鐨勫彉鍖栬繃绋嬩笌瑙傚療鍒扮殑鐢熼暱闃舵鐩稿叧銆傚湪鏁翠釜瀹為獙杩囩▼涓紝鏁ｅ皠鍏変俊鍙风殑骞冲潎鍙樺紓绯绘暟涓?.2%锛屽洜姝ゅ彲鍦ㄥ缓璁殑璁剧疆涓繘琛屽钩琛屽厜钀ュ吇鍩瑰吇銆傜绾挎牱鏈獙璇佷簡鍦ㄧ嚎淇″彿鐨勫噯纭€с€?35灏忔椂鍚庨噰鏍凤紙姝ゆ椂鐢变簬鐭殏鏆撮湶浜庡ぇ姘斾腑鐨凜O2娴撳害涓紝pH鍊煎崌楂橈級锛岀绾縊D750鍊间负40锛岀绾縫H鍊间负7.3锛岃繖涓庡湪绾挎祴瀹氱殑pH鍊?.2楂樺害涓€鑷淬€傚湪瀹為獙缁撴潫鏃讹紝绂荤嚎OD750鍊间负60宸﹀彸锛岃〃鏄庡湪瀹為獙杩囩▼涓煿鍏荤墿鐢熼暱鏃虹洓瀵艰嚧浜х敓杈冮珮鐨勭敓鐗╅噺娴撳害銆侟/p>

缁撹

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鍙剁豢绱犺崸鍏夋护鍏夌墖妯″潡鍦ㄤ綆娴撳害鐢熺墿閲忔祴瀹氫腑鍏锋湁鑹ソ鐨勫垎杈ㄧ巼锛屼笌鍦ㄩ珮缁嗚優瀵嗗害涓嬪噯纭殑鏁ｅ皠鍏夋祴閲忕浉寰楃泭褰?6銆傛澶栵紝鐮旂┒璇佹槑锛屽熀浜嶩PTS鐨刾H娴嬪畾娉曟槸鏇夸唬optode娴嬮噺鐨勭悊鎯宠В鍐虫柟妗堬紝鍗充究鍦ㄨ繛缁収鏄庣殑鏉′欢涓嬶紝璇ユ柟娉曚篃鍚屾牱閫傜敤銆侟/p>

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1. Ru, I.T.K., et al., Chlorella vulgaris: a perspective on its potential for combining high biomass with high value bioproducts.Applied Phycology, 2020. 1(1): p. 2-11.2. Yeh, K.-L. and J.-S. Chang, Effects of cultivation conditions and media composition on cell growth and lipid productivity ofindigenous microalga Chlorella vulgaris ESP-31. Bioresource technology, 2012. 105: p. 120-127.3. Maruyama, I., et al., Application of unicellular algae Chlorella vulgaris for the mass-culture of marine rotifer Brachionus, inLive Food in Aquaculture. 1997, Springer. p. 133-138.4. Chisti, Y., Constraints to commercialization of algal fuels. Journal of biotechnology, 2013. 167(3): p. 201-214.5. Melis, A., Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximizeefficiency. Plant science, 2009. 177(4): p. 272-280.6. Ojo, E.O., et al., Design and parallelisation of a miniature photobioreactor platform for microalgal culture evaluation andoptimisation. Biochemical Engineering Journal, 2015. 103: p. 93-102.7. Hillig, F., et al., Bioprocess Development in Single-Use Systems for Heterotrophic Marine Microalgae. Chemie IngenieurTechnik, 2013. 85(1-2): p. 153-161.8. Morschett, H., et al., Laboratory-scale photobiotechnology-current trends and future perspectives. FEMS Microbiol Lett,2018. 365(1).9. Kiss, B. and 脕. N茅meth, High-throughput microalgae cultivation with adjustable LED-module applying different colours forNannochloropsis and Chlorella microcultures. Acta Alimentaria, 2019. 48(1): p. 115-124.10. Bischoff, H.W. and H.C. Bold, Some soil algae from Enchanted Rock and related algal species. 1963, Austin, Tex.: Universityof Texas.11. Bold, H.C., The Morphology of Chlamydomonas chlamydogama, Sp. Nov. Bulletin of the Torrey Botanical Club, 1949. 76(2):p. 101-108.12. Andersen, R.A., Algal culturing techniques. 2005, Burlington, Mass.: Elsevier/Academic Press.13. Morschett, H., W. Wiechert, and M. Oldiges, Accelerated Development of Phototrophic Bioprocesses: A ConceptualFramework. 2017, RWTH Aachen University.14. Wiltshire, K.H., et al., The determination of algal biomass (as chlorophyll) in suspended matter from the Elbe estuary andthe German Bight: A comparison of high-performance liquid chromatography, delayed fluorescence and promptfluorescence methods. Journal of Experimental Marine Biology and Ecology, 1998. 222(1): p. 113-131.15. Ramaraj, R., D.D. Tsai, and P.H. Chen, Chlorophyll is not accurate measurement for algal biomass. Chiang Mai Journal ofScience, 2013. 40(4): p. 547-555.16. m2p-labs GmbH, Baesweiler Germany, The scattered light signal: Calibration of biomass. 2015.17. Carvalho, A.P., et al., Light requirements in microalgal photobioreactors: an overview of biophotonic aspects. AppliedMicrobiology and Biotechnology, 2011. 89(5): p. 1275-1288.18. Johnson, T.J., et al., Photobioreactor cultivation strategies for microalgae and cyanobacteria. Biotechnology Progress,2018. 34(4): p. 811-827.19. Daliry, S., et al., Investigation of optimal condition for Chlorella vulgaris microalgae growth. Global journal of environmentalscience and management, 2017. 3(2).20. Gong, Q., et al., Effects of Light and pH on Cell Density of Chlorella Vulgaris. Energy Procedia, 2014. 61: p. 2012-2015.21. Sforza, E., et al., Adjusted Light and Dark Cycles Can Optimize Photosynthetic Efficiency in Algae Growing inPhotobioreactors. PLoS ONE, 2012. 7(6): p. e38975.22. Serra-Maia, R., et al., Influence of temperature on Chlorella vulgaris growth and mortality rates in a photobioreactor. AlgalResearch, 2016. 18: p. 352-359.23. Converti, A., et al., Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsisoculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing: Process Intensification,2009. 48(6): p. 1146-1151.24. Minhas, A.K., et al., A Review on the Assessment of Stress Conditions for Simultaneous Production of Microalgal Lipids andCarotenoids. Frontiers in Microbiology, 2016. 7.25. Kong, W., et al., The characteristics of biomass production, lipid accumulation and chlorophyll biosynthesis of Chlorellavulgaris under mixotrophic cultivation. African Journal of Biotechnology, 2011. 10(55): p. 11620-11630.26. Scarsella, M., et al., Study on the optimal growing conditions of Chlorella vulgaris in bubble column photobioreactors.Chem. Eng, 2010. 20: p. 85-90.27. Heredia-Arroyo, T., et al., Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulationfrom non-sugar materials. Biomass and Bioenergy, 2011. 35(5): p. 2245-2253.28. Blair, M.F., B. Kokabian, and V.G. Gude, Light and growth medium effect on Chlorella vulgaris biomass production. Journalof Environmental Chemical Engineering, 2014. 2(1): p. 665-674.29. Keil, T., et al., Polymer-based ammonium-limited fed-batch cultivation in shake flasks improves lipid productivity of themicroalga Chlorella vulgaris. Bioresour Technol, 2019. 291: p. 121821.30. Hallmann, A., Algae biotechnology鈥揼reen cell-factories on the rise. Current Biotechnology, 2015. 4(4): p. 389-415.31. Fayyaz, M., et al., Genetic engineering of microalgae for enhanced biorefinery capabilities. Biotechnology Advances, 2020.43: p. 107554.32. Ng, I.S., et al., Recent Developments on Genetic Engineering of Microalgae for Biofuels and Bio-Based Chemicals.Biotechnology Journal, 2017. 12(10): p. 1600644.